Team:ESBS-Strasbourg/Parts

From 2008.igem.org

(Difference between revisions)
(Toolbox for modular transcription factors)
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==Toolbox for modular transcription factors==
==Toolbox for modular transcription factors==
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[[image:ESBS_TF.jpg|thumb|right]]
===DNA binding domains===
===DNA binding domains===
* '''tetR''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
* '''tetR''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
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==Toolbox for modular promoters==
==Toolbox for modular promoters==
===Minimal promoters of different strength===
===Minimal promoters of different strength===

Revision as of 23:00, 29 October 2008


Parts submitted

Following you can find a summary of our contribution to the registry.

Toolbox for modular transcription factors

ESBS TF.jpg

DNA binding domains

  • tetR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
  • cI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
  • lexA [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
  • lacI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
  • Gal4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]

Activation domain

  • VP16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]

Repression domain

  • TUP1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]

Fluorescent markers

  • CFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
  • mCherry [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
  • mOrange [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]

Junctions

  • linker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]

Tags

  • cin8 - cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
  • hsl1 – cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]


=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]

Toolbox for modular promoters

Minimal promoters of different strength

  • cyc100 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
  • cyc70 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
  • cyc43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
  • cyc28 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
  • cyc16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]

Operator sites

  • tetR operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
  • cI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
  • lexA operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
  • lacI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
  • Gal4 operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]


Yeast selection markers

  • Leu2 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
  • Ura3 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]


Remakes of good, but not available BioBricks

  • Gal1 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
  • Adh1 terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]