Parts submitted

Here you can find a little summary of our contribution to the registry:

Toolbox for modular transcription factors

DNA binding domains

• tetR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
• cI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
• lexA [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
• lacI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
• Gal4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]

Activation domain

• VP16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]

Repression domain

• TUP1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]

Fluorescent markers

• CFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
• mCherry [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
• mOrange [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]

Junctions

• linker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]

Tags

• cin8 - cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
• hsl1 – cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]

=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]

Toolbox for modular promoters

Minimal promoters of different strength

• cyc100 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
• cyc70 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
• cyc43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
• cyc28 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
• cyc16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]

Operator sites

• tetR operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
• cI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
• lexA operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
• lacI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
• Gal4 operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]

Yeast selection markers

• Leu2 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
• Ura3 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]

Remakes of good, but not available BioBricks

• Gal1 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
• Adh1 terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]