Team:ESBS-Strasbourg/Protocols

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Protocols

E.Coli

Preparation of competent cells (Barbara's Protocol)
[http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)]
Transformation of competent cells (Barbara's Protocol)
Heat shock transformation

Cloning

Solutions and buffers

[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells]
[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes]
TENS for the do it yourself MiniPrep

Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]

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