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Team:ESBS-Strasbourg/Protocols
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Revision as of 00:13, 29 October 2008 by
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home
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Meet the Team
CoLABorations
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project
Summary
In Detail
System
Labeling
Measurements
Outcome
Modeling
Related
Applications
Brainstorming
Reading
misc
Links
Protocols
Tutorials + Papers
parts
Submitted Parts
Sandbox
notebook
Protocols
E.Coli
Preparation of competent cells (Barbara's Protocol)
Preparation of TOP10 chemically competent cells (iGEM)
Transformation of competent cells (Barbara's Protocol)
Heat shock transformation
Cloning
PCR
Purification of PCR products
Miniprep:Plasmid Amplification
Plasmid Purification
Restriction Digestion
Agarose Gel Preparation
Dephosphorylation
Ligation
Concentrations Measurements
Solutions and buffers
SOB for the preparation of competent cells
SOC for bacterial transformation purposes
TENS for the do it yourself MiniPrep
Further source for protocols:
Protocol-online.org
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