Team:Harvard/Parts/Tempsenseci
From 2008.igem.org
(→Thermoinducible cI System) |
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This system uses a a temperature sensitive variant of cI lambda to regulate the lambda promoter. | This system uses a a temperature sensitive variant of cI lambda to regulate the lambda promoter. | ||
- | The thermoinducible cI lambda system uses cI857 (a mutant form of cI from [http://www.addgene.org/pgvec1?f=c&vectorid=5079&cmd=genvecmap&dim=800&format=html&mtime=1188314819 | + | The thermoinducible cI lambda system uses cI857 (a mutant form of cI from [http://www.addgene.org/pgvec1?f=c&vectorid=5079&cmd=genvecmap&dim=800&format=html&mtime=1188314819 pGW7] purchased from [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx#40554 ATCC]) to regulate expression of genes under the control of the lambda promoter. The cI857 repressor is repressed by thermal denaturation. Activity of cI857 begins to decrease around 30 ºC and is fully denatured by around 42 ºC (Leipold et al., 1994). Thus transcription of the gene under the control of the lambda promoter can be induced by increasing the temperature from 30 ºC to 37 ºC-40 ºC. |
- | We had previously tried to use the thermoinducible lac system in the Registry ([http://partsregistry.org/Part:BBa_J06912 BBa_J06912] and [http://partsregistry.org/Part:BBa_J06911 BBa_J06911]). However, induction tests, a [https://2008.igem.org/Team:Harvard/Dailybook/Week6/Chemical_and_Light#Western_Blot | + | We had previously tried to use the thermoinducible lac system in the Registry ([http://partsregistry.org/Part:BBa_J06912 BBa_J06912] and [http://partsregistry.org/Part:BBa_J06911 BBa_J06911]). However, induction tests, a [https://2008.igem.org/Team:Harvard/Dailybook/Week6/Chemical_and_Light#Western_Blot Western blot], and sequencing confirmed that these parts are not functional. We thus focused our efforts on creating a thermosensitive cI system. |
===BBa_K098995=== | ===BBa_K098995=== |
Revision as of 03:01, 30 October 2008
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