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Team:Hawaii/Notebook/2008-06- 9
From 2008.igem.org
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| - | + | = Things we did today = | |
| - | === | + | ==Wetlab Work== |
| - | + | === Practiced getting DNA plasmids off the filter paper === | |
| + | :<strong>Norman and Krystle</strong> | ||
| + | :*see experiment for details | ||
| + | :DNA will be frozen in TE for later transformation | ||
:: BBa_E0040 GFP biobrick (spot 7E on plate 1001) | :: BBa_E0040 GFP biobrick (spot 7E on plate 1001) | ||
:: BBa_J04430 IPTG-induced GFP device (spot 4H on plate 1004) | :: BBa_J04430 IPTG-induced GFP device (spot 4H on plate 1004) | ||
:: BBa_I13522 constitutive GFP device (spot 8A on plate 1002) | :: BBa_I13522 constitutive GFP device (spot 8A on plate 1002) | ||
| - | === | + | === Made Spectinomycin/Streptomycin Plates === |
| - | * Made 1000X sp stock solution | + | :<strong>Grace</strong> |
| - | * Made 1000X sm stock solution | + | :*Made 1000X sp stock solution |
| - | * Made BG-11+sp+sm plates | + | :*Made 1000X sm stock solution |
| - | * Made LB+sp+sm plates | + | :*Made BG-11+sp+sm plates |
| - | * Made LB+sp plates | + | :*Made LB+sp+sm plates |
| - | + | :*Made LB+sp plates | |
| + | |||
| + | ===Transformed BioBricks into competent DH5α=== | ||
| + | : <strong>Grace</strong> | ||
| + | :* see experiment for details | ||
| + | |||
| + | ==Drylab Work== | ||
| + | |||
== Discussion == | == Discussion == | ||
Revision as of 07:51, 10 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab Work
Practiced getting DNA plasmids off the filter paper
- Norman and Krystle
- see experiment for details
- DNA will be frozen in TE for later transformation
- BBa_E0040 GFP biobrick (spot 7E on plate 1001)
- BBa_J04430 IPTG-induced GFP device (spot 4H on plate 1004)
- BBa_I13522 constitutive GFP device (spot 8A on plate 1002)
Made Spectinomycin/Streptomycin Plates
- Grace
- Made 1000X sp stock solution
- Made 1000X sm stock solution
- Made BG-11+sp+sm plates
- Made LB+sp+sm plates
- Made LB+sp plates
Transformed BioBricks into competent DH5α
- Grace
- see experiment for details
Drylab Work
Discussion
Quote of the Day
"So that's the difference between biochemists and geneticists...[http://manoa.hawaii.edu/
- Biochemists will be so meticulous and do everything the long way.
- Geneticists will try to look for a shortest, simplest possible."
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
