Team:Hawaii/Notebook/2008-07-18

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Revision as of 23:21, 19 July 2008 by MargaretRuzicka (Talk | contribs)
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Things we did today

Wetlab work

PCR of pRL1383a Parts

Margaret
  • for details concerning the running conditions, please look here.
  • Ran a 0.8%gel at 95V.
    File:PCR 7 18 08
    Please refer to the annotated picture.

Extraction of Biobricks and Transformation in DB3.1

Margaret
  • streaked plates on Amp100 plates
  • for details please look here.

Sequencing

Grace
  • Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
  • Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
  • Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
  • Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
  • Treated PCR products w/ ExoSAP
  • Determined DNA concentrations using nanodrop spectrometer (measured twice)
DNA concentrations of PCR products
PCR Sample Concentration (1st measurement) Concentration (2nd measurement)
nir 1015 ng/μl 1010 ng/μl
slr2016-1 1295 ng/μl 1107 ng/μl
slr2016-2 1518 ng/μl1266 ng/μl
pilA 1312 ng/μl1330 ng/μl
B0024 1546 ng/μl1474 ng/μl
B0034 1537 ng/μl1386 ng/μl
C0012 2130 ng/μl1530 ng/μl
E0040 1502 ng/μl1236 ng/μl
J33207 1454 ng/μl1223 ng/μl
BB-pRL1383a 2279 ng/μl2129 ng/μl
GFP fusion 1660 ng/μl1714 ng/μl
  • Sent 22 samples to CORE Hawaii for sequencing

Media Making

Margaret, Krystle (thanks for the help!)

  • Made one sleeve of Amp100 plates.

Drylab Work

Project Description (Abstract)

Grace, Krystle, Margaret
  • Wrote description of project (abstract)

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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