Team:Hawaii/Notebook/2008-07- 1

From 2008.igem.org

(Difference between revisions)
(→Discussion)
(→Plasmid prep)
 
Line 21: Line 21:
:<strong> Grace</strong>
:<strong> Grace</strong>
-
:* Took 1 ml of the overnight Biobrick cultures to inoculate 100 ml LB+amp<sub>100</sub>
+
:* Took 1 ml of the overnight Biobrick cultures to inoculate 100 ml LB+amp<sub>100</sub>. Incubated 8 hours at 37C with shaking (150 rpm). Stored in 4C until tomorrow morning.
===Biobrick extraction===
===Biobrick extraction===

Latest revision as of 06:13, 2 July 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

PCR gel

Margaret
  • Ran 1% gel for oriV, rep, mob, and omega PCR products

Glycerol stocked Biobricks

Grace
  • Glycerol stocked B0030, C0012, E0040, J33207, and R0010

Made LB liquid media

Grace
  • Two 750ml batches

Plasmid prep

Grace
  • Took 1 ml of the overnight Biobrick cultures to inoculate 100 ml LB+amp100. Incubated 8 hours at 37C with shaking (150 rpm). Stored in 4C until tomorrow morning.

Biobrick extraction

Grace
  • B0014 (double terminator), plated on LB+amp50+kan20
  • B0015 (double terminator), plated on LB+amp50+kan20
  • E0026 (enhanced cyan fluorescent protein), plated on LB+amp100
  • E0240 (GFP device), plated on LB+amp100
  • I14032 (lac promoter), plated on LB+kan50
  • J04430 (IPTG induced GFP device), plated on LB+amp100

Drylab Work

Project description write-ups

Margaret & Grace
  • Updated project descriptions on wiki

Secretion project presentation

Krystle & Grace
  • Worked on powerpoint and flowchart for Thursday's meeting

Plans for the rest of the week

Margaret, Krystle, Grace, and Norman
  • Tomorrow
  • Redo PCR and run on gel (MR)
  • Plasmid prep for B0030, C0012, E0040, J33207, and R0010 (MR)
  • Restock cyanos (KS)
  • Restreak any transformed Biobrick plates from today (GK)
  • Thursday
  • Organize cryostocks (GK)

Discussion

  • FYI: Spectrophotometer broken. Make arrangements to use someone else's spectrophotometer if needed.

Quote of the Day

GK: "It depends on if we're dinnering tomorrow."
KS: "Since when did dinner become a verb?"


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


Retrieved from "http://2008.igem.org/Team:Hawaii/Notebook/2008-07-_1"