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Team:Hawaii/Notebook/2008-08-10
From 2008.igem.org
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== Wetlab work == | == Wetlab work == | ||
===Checked DNA products via gel electrophoresis=== | ===Checked DNA products via gel electrophoresis=== | ||
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[[Image:081008REdigest.jpg|right|thumb|200px|EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.]] | [[Image:081008REdigest.jpg|right|thumb|200px|EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.]] | ||
| - | + | :<strong> Grace</strong> | |
:* Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min. | :* Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min. | ||
::* Picture really dark. Adjust camera? Restain with more EtBr tomorrow? | ::* Picture really dark. Adjust camera? Restain with more EtBr tomorrow? | ||
Revision as of 07:45, 11 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Checked DNA products via gel electrophoresis
- Grace
- Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min.
- Picture really dark. Adjust camera? Restain with more EtBr tomorrow?
- Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour
- Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
- Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
