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Team:Hawaii/Notebook/2008-08-10
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Checked DNA products via gel electrophoresis=== :<strong> Grace</strong> :* Ran RE digests on an EtBr stained 2% agaro...) |
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== Wetlab work == | == Wetlab work == | ||
===Checked DNA products via gel electrophoresis=== | ===Checked DNA products via gel electrophoresis=== | ||
| + | [[Image:081008REdigest.jpg|right|thumb|200px|EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.]] | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | + | :* Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min. | |
| - | :* Ran RE digests on an EtBr stained 2% agarose gel at 72V for | + | ::* Picture really dark. Adjust camera? Restain with more EtBr tomorrow? |
:* Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour | :* Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour | ||
::* Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present. | ::* Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present. | ||
| - | + | ::* Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow. | |
| + | ===Cryostocks=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* ''E. coli'' did not grow. Reinoculate tomorrow. | ||
= Discussion = | = Discussion = | ||
Latest revision as of 04:03, 12 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Checked DNA products via gel electrophoresis
- Grace
- Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min.
- Picture really dark. Adjust camera? Restain with more EtBr tomorrow?
- Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour
- Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
- Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.
Cryostocks
- Grace
- E. coli did not grow. Reinoculate tomorrow.
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
