Latest revision as of 04:03, 12 August 2008

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Things we did today

EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.

Grace

Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour

Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.

Grace

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 4: / Line 4: @@
 == Wetlab work ==
 ===Checked DNA products via gel electrophoresis===
+[[Image:081008REdigest.jpg|right|thumb|200px|EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.]]
 :<strong> Grace</strong>
 :* Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min.
 ::* Picture really dark. Adjust camera? Restain with more EtBr tomorrow?
@@ Line 11: / Line 11: @@
 ::* Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
 ::* Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.
-[[Image:081008REdigest.jpg|left|thumb|400px|EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.]][[Image:081008plasmidprep.jpg|right|thumb|400px|EtBr stained 1.2% agarose gel ran at 95V for 60 min. Five microliters of the plasmid preps were loaded into each lane.]]
+===Cryostocks===
+:<strong>Grace</strong>
+:* ''E. coli'' did not grow. Reinoculate tomorrow.
 = Discussion =