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Team:Hawaii/Notebook/2008-08-10
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Checked DNA products via gel electrophoresis
- Grace
- Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min.
- Picture really dark. Adjust camera? Restain with more EtBr tomorrow?
- Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour
- Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
- Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.
File:081008plasmidprep.jpg
EtBr stained 1.2% agarose gel ran at 95V for 60 min. Five microliters of the plasmid preps were loaded into each lane.
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
