Team:Hawaii/Notebook/2008-08-20

From 2008.igem.org

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Things we did today

Wetlab work

Name of Task

Annealing was quite successful in that there is only one BRIGHT band.
Margaret & Krystle
  • Annealed and phosphorylated:
  • plac (I14032) for front ligation
  • rbs 1.0 (B0034) for back ligation
  • rbs 0.6 (B0030) for back ligation
  • rbs 0.6 (B0030) for front ligation
  • tt (B1006) for back ligation
  • We decided to perform this reaction again with out gel purification and the gel was discarded.
  • These annealed products can be found in the -20°C freezer in the DNA box.
  • Quantification was achieved simply by adding up the amount of DNA added.
  • plac (I14032) for front ligation --> 370ng/ul
  • rbs 1.0 (B0034) for back ligation --> 206.7ng/ul
  • rbs 0.6 (B0030) for front ligation -->
  • rbs 0.6 (B0030) for back ligation --> 228.3ng/ul
  • tt (B1006) for back ligation --> 375ng/ul

Restriction Digest

Krystle
  • B0034 with EcoRI and XbaI (for ligation with plac)
  • nir with SpeI and PstI (for ligation with RBS)
  • gfp wih SpeI and PstI (for ligation with TT)
  • gfp with EcoRI and XbaI (for ligation with RBS)

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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