From 2008.igem.org

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Things we did today

Wetlab work

Construction of p+r and rereplacement of BB-pRL1383a MCS

Grace

EtBr stained 2% (L) and 0.8% (R) agarose gels ran at 60V for 2 hours and 1 hour, respectively. Thirty microliters of RE digest reaction were loaded into each well.

• Ran RE digests from last night on agarose gel
• Extracted bands from gel
• Ligated:

• BB-pRL1383a with J33207
• nir + rbs (B0034)
• plac + rbs (B0034)

• RE digested p+r with EcoRI, XbaI, SpeI, PstI

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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