Revision as of 19:11, 27 September 2008

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Things we did today

Grace

EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Two microliters of PCR product were loaded into each well.

Construct	Colonies
nir+rbs+GFP + tt	3
nir + rbs+GFP+tt1	1
plac + rbs+GFP+tt1	1
nir + rbs+GFP+tt2	5
plac + rbs+GFP+tt2	0
pSB1A3 to self (- control)	0
J33207 + pRL1383a (plated on IPTG+Xgal)	31
J33207 + pRL1383a (1:10) (plated on Xgal)	0
pRL1383a to self (+ control)	0
J33207 plasmid (+ control, plated on IPTG+Xgal)	162 (all blue!)
pRL1383a plasmid (+ control)	0
no DNA	0

Margaret, Krystle, Grace

Reviewed Margaret's presentation for Dr. Lee and outlined presentation for Boston.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 29: / Line 29: @@
 |-
 |align=center|J33207 + pRL1383a (plated on IPTG+Xgal)
-|align=center|
+|align=center|31
 |-
 |align=center|J33207 + pRL1383a (1:10) (plated on Xgal)
@@ Line 47: / Line 47: @@
 |}
 :* Colony PCR of transformants
+::* Bands too small for all secretion constructs
+::* BB-pRL1383a bands all ~875 (expected size = 850bp). :D!
+:* Restreaked BB-pRL1383a colonies #1, 3, 13, 18
 == Drylab Work ==