Team:Hawaii/Notebook/2008-09-26

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Things we did today

Wetlab work

Verification of Transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Two microliters of PCR product were loaded into each well.
Construct Colonies
nir+rbs+GFP + tt 3
nir + rbs+GFP+tt1 1
plac + rbs+GFP+tt1 1
nir + rbs+GFP+tt2 5
plac + rbs+GFP+tt2 0
pSB1A3 to self (- control) 0
J33207 + pRL1383a (plated on IPTG+Xgal)
J33207 + pRL1383a (1:10) (plated on Xgal) 0
pRL1383a to self (+ control) 0
J33207 plasmid (+ control, plated on IPTG+Xgal) 162 (all blue!)
pRL1383a plasmid (+ control) 0
no DNA 0
  • Colony PCR of transformants

Drylab Work

iGEM presentation

Margaret, Krystle, Grace
  • Reviewed Margaret's presentation for Dr. Lee and outlined presentation for Boston.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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