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Team:Hawaii/Notebook/2008-09- 8
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Inoculated for plasmid prep and cryostock=== :<strong> Grace</strong> :* nir+rbs :* rbs+GFP :* plac+rbs :* slr1+GFPf :...) |
(→Wetlab work) |
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:* BB-pRL plates | :* BB-pRL plates | ||
| + | ===PCR=== | ||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :*20ul reactions of nir, pilA, slr1 using VF2 and VR primers | ||
| + | :*10ul reaction plac +rbs using VF2 and rbs specific primer | ||
= Discussion = | = Discussion = | ||
Latest revision as of 04:04, 12 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Inoculated for plasmid prep and cryostock
- Grace
- nir+rbs
- rbs+GFP
- plac+rbs
- slr1+GFPf
- pilA+GFPf (8/12 and 8/13 transformations)
Restreaked to purify colonies
- Grace
- 3A: plac+rbs colony #10
- BB-pRL plates
PCR
- Krystle
- 20ul reactions of nir, pilA, slr1 using VF2 and VR primers
- 10ul reaction plac +rbs using VF2 and rbs specific primer
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
