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Team:Hawaii/Notebook/2008-10-11
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(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Insertion of secretion device into BBpRL1383a=== :<strong> Grace</strong> [[Image:101108REdigest.jpg|right|thumb|400px|...) |
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== Wetlab work == | == Wetlab work == | ||
===Insertion of secretion device into BBpRL1383a=== | ===Insertion of secretion device into BBpRL1383a=== | ||
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[[Image:101108REdigest.jpg|right|thumb|400px|EtBr stained 0.8% agarose gel ran at 60V for 75 min. Thirty microliters of RE digested PCR product and twenty microliters of RE digested plasmid prep were loaded into each well.]] | [[Image:101108REdigest.jpg|right|thumb|400px|EtBr stained 0.8% agarose gel ran at 60V for 75 min. Thirty microliters of RE digested PCR product and twenty microliters of RE digested plasmid prep were loaded into each well.]] | ||
| + | :<strong> Grace</strong> | ||
:* Ran RE digests on gel | :* Ran RE digests on gel | ||
:* Extracted bands from gel | :* Extracted bands from gel | ||
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:<strong>Krystle</strong> | :<strong>Krystle</strong> | ||
:* Transformed into DH5α cells | :* Transformed into DH5α cells | ||
| + | |||
= Discussion = | = Discussion = | ||
Latest revision as of 07:09, 22 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Insertion of secretion device into BBpRL1383a
- Grace
- Ran RE digests on gel
- Extracted bands from gel
- Ligated constructs with BBpRL1383a
- Krystle
- Transformed into DH5α cells
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
