Team:Hawaii/Notebook/2008-10-11

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Things we did today

Wetlab work

Insertion of secretion device into BBpRL1383a

EtBr stained 0.8% agarose gel ran at 60V for 75 min. Thirty microliters of RE digested PCR product and twenty microliters of RE digested plasmid prep were loaded into each well.
Grace
  • Ran RE digests on gel
  • Extracted bands from gel
  • Ligated constructs with BBpRL1383a
Krystle
  • Transformed into DH5α cells

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


Sponsor_UHM.gifSponsor_OVCRGE.gifSponsor_CTAHR.gif


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