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Things we did today

Wetlab work

Insertion of secretion device into BBpRL1383a

EtBr stained 0.8% agarose gel ran at 60V for 75 min. Thirty microliters of RE digested PCR product and twenty microliters of RE digested plasmid prep were loaded into each well.

Grace

• Ran RE digests on gel
• Extracted bands from gel
• Ligated constructs with BBpRL1383a

Krystle

• Transformed into DH5α cells

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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