"
Team:Hawaii/Notebook/2008-10-22
From 2008.igem.org
(Difference between revisions)
(→Extracted J04430 from filter paper) |
(→Wetlab work) |
||
| Line 19: | Line 19: | ||
:* Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1 | :* Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1 | ||
| + | |||
| + | ===Construction of GFP secretion device=== | ||
| + | :<strong>Krystle</strong> | ||
| + | :* Gel purify restriction digests from yesterday | ||
| + | :* Ligate nir+rbs and slr+gfpf into restricted psB1A3 from Margaret | ||
| + | :* Transformed into DH5α | ||
= Discussion = | = Discussion = | ||
Latest revision as of 01:06, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep of BB-pRL1383a
- Grace
- E. coli did not grow. Reinoculated.
Extracted J04430 from filter paper
- Grace
- Used 2 μl of filter paper solution for PCR to verify plasmid
- Something is wrong with the J04430 filter spot iGEM sent us. PCR with VF2/VR primers did NOT yield the expected band ~1000bp.
Transformation
- Grace
- Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1
Construction of GFP secretion device
- Krystle
- Gel purify restriction digests from yesterday
- Ligate nir+rbs and slr+gfpf into restricted psB1A3 from Margaret
- Transformed into DH5α
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
