"
Team:Hawaii/Notebook/2008-10- 7
From 2008.igem.org
(Difference between revisions)
(→Discussion) |
(→Construction of secretion device (cont.)) |
||
| (2 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
===Construction of secretion device (cont.)=== | ===Construction of secretion device (cont.)=== | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | + | [[Image:100708REdigest.png|right|thumb|500px|EtBr stained 2% agarose gel ran at 60V for 150 minutes. Thirty microliters of RE digest reaction were loaded into each well.]] | |
:* Ran RE digests on gel | :* Ran RE digests on gel | ||
| + | ::* J33207 did not cut | ||
:* Extracted bands from gel | :* Extracted bands from gel | ||
:* Ligated: | :* Ligated: | ||
| Line 17: | Line 18: | ||
::* pSB1A3 to self (control) | ::* pSB1A3 to self (control) | ||
:* Used 5 μl ligation mixture to transform DH5α cells | :* Used 5 μl ligation mixture to transform DH5α cells | ||
| - | |||
==Drylab Work== | ==Drylab Work== | ||
Latest revision as of 06:54, 22 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on gel
- J33207 did not cut
- Extracted bands from gel
- Ligated:
- nir + rbs+gfp+tt#1
- plac + rbs+gfp+tt#1
- nir+rbs + slr1+GFPf
- plac+rbs + slr1+GFPf
- nir+rbs + pilA+GFPf+tt
- plac+rbs + pilA+GFPf+tt
- pSB1A3 to self (control)
- Used 5 μl ligation mixture to transform DH5α cells
Drylab Work
- Grace, Krystle, Margaret
- Revised project abstract
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
