Team:Hawaii/Notebook/2008-10- 7
From 2008.igem.org
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on gel
- J33207 did not cut
- Extracted bands from gel
- Ligated:
- nir + rbs+gfp+tt#1
- plac + rbs+gfp+tt#1
- nir+rbs + slr1+GFPf
- plac+rbs + slr1+GFPf
- nir+rbs + pilA+GFPf+tt
- plac+rbs + pilA+GFPf+tt
- pSB1A3 to self (control)
- Used 5 μl ligation mixture to transform DH5α cells
Drylab Work
- Grace, Krystle, Margaret
- Revised project abstract
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]