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Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C)) |
(→Parts stored in the fridge in the lab (4°C)) |
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|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI||+/- 30||133.1||21||July 29 | |[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI||+/- 30||133.1||21||July 29 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006(Ap)||3d lock||+/- | + | |[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006(Ap)||3d lock||+/- 35||117.4||23||July 29 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006(Ap)||3d lock||+/- 39.5||144.8||24||July 29 | |[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006(Ap)||3d lock||+/- 39.5||144.8||24||July 29 | ||
| Line 65: | Line 65: | ||
|[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006(Ap)||3d key+ terminator||+/- 40||160.7||25||July 29 | |[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006(Ap)||3d key+ terminator||+/- 40||160.7||25||July 29 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006(Ap)||3d key+ terminator||+/- | + | |[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006(Ap)||3d key+ terminator||+/- 46||308.3||29||July 29 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_B0015 B0015]||BBa_J52017(Ap,Km)||Terminator||+/- 48||311.0||31||July 29 | |[http://partsregistry.org/Part:BBa_B0015 B0015]||BBa_J52017(Ap,Km)||Terminator||+/- 48||311.0||31||July 29 | ||
| Line 101: | Line 101: | ||
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||33.4||3.8||3||July 24 | |[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||33.4||3.8||3||July 24 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779|| | + | |[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||22||9.5||4||July 24 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52|| | + | |[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52||21||4.2||5||July 29 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||28||6.1||6||July 29 | |[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||28||6.1||6||July 29 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||62|| | + | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||62||25||3.4||7||July 29 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090|| | + | |[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090||2||12.9||8||July 30 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090||45||1.3||9||July 31 | |[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090||45||1.3||9||July 31 | ||
| Line 115: | Line 115: | ||
|[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Xba'' I||Vector||2077||20||4.0||10||July 31 | |[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Xba'' I||Vector||2077||20||4.0||10||July 31 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303|| | + | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||31||11.7||11||July 31 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||12||July 30 | |[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||12||July 30 | ||
| Line 121: | Line 121: | ||
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||7.5||13||July 30 | |[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||7.5||13||July 30 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774|| | + | |[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774||18||24.7||14||August 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0060 C0060]|| ''Eco''R I||''Spe'' I||Insert||806||15||8.2||15||August 01 | |[http://partsregistry.org/Part:BBa_C0060 C0060]|| ''Eco''R I||''Spe'' I||Insert||806||15||8.2||15||August 01 | ||
Revision as of 08:18, 6 August 2008
dock/undock dropdown
Parts stored in the fridge in the lab (4°C)
| Plasmids: MiniPrep Products | ||||||
|---|---|---|---|---|---|---|
| Part | Plasmid | Description | µl left | ng/µl | Box | Date |
| M30109 | pSB1AC3 | Light-sensor | +/- 40 | 50.4 | 1 | July 23 |
| M30109 | pSB1AC3 | Light-sensor | +/- 30 | 54.3 | 2 | July 24 |
| J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 60 | 345.0 | 3 | July 24 |
| J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 30 | 176.1 | 4 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 5 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 6 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 7 | July 29 |
| C0040 | pSB1A2 | TetR repressor | +/- 25 | 177.1 | 8 | July 24 |
| C0040 | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 9 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 40 | 63.0 | 10 | July 23 |
| B0015 | pSB1AK3 | Terminator | +/- 10 | 112.3 | 11 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 20 | 130.3 | 12 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 45 | 303.1 | 13 | July 29 |
| B0015 | pSB1AK3 | Terminator | +/- 30 | 213.6 | 14 | July 29 |
| R0084 | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 15 | July 23 |
| R0084 | pSB1A2 | OmpF promoter | +/- 30 | 60.9 | 16 | July 24 |
| B0032 | pSB1A2 | RBS (efficiency 0.3) | +/- 10 | 114.8 | 18 | July 24 |
| E0022 | pSB1A2 | eCFP | +/- 40 | 84.2 | 19 | July 23 |
| E0022 | pSB1A2 | eCFP | +/- 40 | 86.4 | 20 | July 24 |
| C0012 | pSB1A2 | LacI | +/- 30 | 133.1 | 21 | July 29 |
| J23032 | BBa_J23006(Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
| J23032 | BBa_J23006(Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
| J23032 | BBa_J23006(Ap) | 3d lock | +/- 48 | 129.1 | 28 | July 29 |
| J23022 | BBa_J23006(Ap) | 3d key+ terminator | +/- 40 | 160.7 | 25 | July 29 |
| J23022 | BBa_J23006(Ap) | 3d key+ terminator | +/- 46 | 308.3 | 29 | July 29 |
| B0015 | BBa_J52017(Ap,Km) | Terminator | +/- 48 | 311.0 | 31 | July 29 |
| J23109 | BBa_J61002(Ap) | Constitutive Promotor | +/- 46 | 375.1 | 26 | July 29 |
| J23109 | BBa_J61002(Ap) | constitutive Pr | +/- 48 | 404 | 30 | July 29 |
| I712074 | BBa_J52017(Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 27 | July 29 |
| F1610 | pSB1AK3 | RBS + LuxI + Terminator | +/- 30 | 104.0 | 22 | July 29 |
| R0062 | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 37 | July 31 |
| Restriction Digests | ||||||||
|---|---|---|---|---|---|---|---|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
| J23100 | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 1 | July 24 |
| C0040 | EcoR I | Spe I | Insert | 677 | 25 | 7.1 | 2 | July 24 |
| R0084 | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 3 | July 24 |
| E0022 | EcoR I | Spe I | Insert | 779 | 22 | 9.5 | 4 | July 24 |
| J23109 | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 5 | July 29 |
| R0084 | EcoR I | Spe I | Insert | 125 | 28 | 6.1 | 6 | July 29 |
| I712074 | EcoR I | Spe I | Insert | 62 | 25 | 3.4 | 7 | July 29 |
| J23032 | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 8 | July 30 |
| J23032 | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 9 | July 31 |
| B0032 | EcoR I | Xba I | Vector | 2077 | 20 | 4.0 | 10 | July 31 |
| B0015 | EcoR I | Xba I | Vector | 3303 | 31 | 11.7 | 11 | July 31 |
| R0011 | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 12 | July 30 |
| R0011 | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 13 | July 30 |
| C0062 | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 14 | August 01 |
| C0060 | EcoR I | Spe I | Insert | 806 | 15 | 8.2 | 15 | August 01 |
| pSB1A2 | EcoR I | Spe I | Plasmid | 2079 | 20 | 18.2 | 16 | August 01 |
| P1010 | EcoR I | Spe I | Insert | 682 | 10 | 4.9 | 17 | August 01 |
