Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C))
(→Parts stored in the fridge in the lab (4°C))
Line 137: Line 137:
|[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 30||60.9||16||July 24
|[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 30||60.9||16||July 24
|}
|}
 +
 +
{|
{|
Line 192: Line 194:
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 20||58.3||7A||August 20
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 20||58.3||7A||August 20
|}
|}
 +
 +
{|
{|
Line 206: Line 210:
|width=90| '''Box'''
|width=90| '''Box'''
|width=90| '''Date'''
|width=90| '''Date'''
-
|- style="background: #E0FFFF; text-align: center;"
 
-
|[http://partsregistry.org/Part:BBa_J23100 J23100]||''Eco''R I||''Spe'' I||Insert||52||38.1||2.6||1||July 24
 
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
 +
|[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||11||11.7||11||July 31
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||10||13.6||17||August 26
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Xba'' I||Vector||2092||5||17.6||25||August 19
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|[http://partsregistry.org/Part:BBa_B0033 B0033]|| ''Eco''R I||''Xba'' I||Vector||2092||20||10.9||39||August 27
 +
|- style="background: #E0FFFF; text-align: center;"
|[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Eco''R I||''Spe'' I||Insert||677||18||7.1||2||July 24
|[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Eco''R I||''Spe'' I||Insert||677||18||7.1||2||July 24
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||33.4||3.8||3||July 24
+
|[http://partsregistry.org/Part:BBa_C0060 C0060]|| ''Eco''R I||''Spe'' I||Insert||803||28||5.4||20||August 12
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||22||9.5||4||July 24
+
|[http://partsregistry.org/Part:BBa_C0060 C0060]|| ''Eco''R I||''Spe'' I||Insert||806||9||8.2||15||August 01
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52||21||4.2||5||July 29
+
|[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774||18||24.7||14||August 01
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||25||6.1||6||July 29
+
|[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774||25||8.3||44||August 27
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||62||23||3.4||7||July 29
+
|[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774||28||5.0||10||August 12
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||22||9.5||4||July 24
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_F1610 F1610]|| ''Eco''R I||''Xba'' I||Vector||3956||26||4.5||35||August 19
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090||2||12.9||8||July 30
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090||2||12.9||8||July 30
Line 226: Line 240:
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3308||25||5.1||22||August 07
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3308||25||5.1||22||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|[http://partsregistry.org/Part:BBa_J23100 J23100]||''Eco''R I||''Spe'' I||Insert||52||38.1||2.6||1||July 24
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||11||11.7||11||July 31
+
|[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52||21||4.2||5||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_C0060 C0060]|| ''Eco''R I||''Spe'' I||Insert||803||28||5.4||20||August 12
+
|[http://partsregistry.org/Part:BBa_J23116 J23116]|| ''Eco''R I||''Spe'' I||Insert||52||+/-20||8.1||26||August 12
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||12||July 30
+
|[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||14.1||27||August 12
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||7.5||13||July 30
+
|[http://partsregistry.org/Part:BBa_K145001 K145001]|| ''Eco''R I||''Spe'' I||Insert||2730||+/-21||24.3||24||August 22
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|[http://partsregistry.org/Part:BBa_K1450151 K145151]|| ''Eco''R I||''Spe'' I||Insert||306||19||25.2||36||August 19
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774||18||24.7||14||August 01
+
|[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2079||9||18.2||16||August 01
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_C0060 C0060]|| ''Eco''R I||''Spe'' I||Insert||806||9||8.2||15||August 01
+
|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2056||24||27.7||43||August 25
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2079||9||18.2||16||August 01
+
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||12||July 30
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_P1010 P1010]|| ''Eco''R I||''Spe'' I||Insert||682||10||4.9||17||August 01
+
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||7.5||13||July 30
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||25||2.1||21||August 27
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0062 R0062]|| ''Eco''R I||''Spe'' I||Insert||55||27||7.8||23||August 07
|[http://partsregistry.org/Part:BBa_R0062 R0062]|| ''Eco''R I||''Spe'' I||Insert||55||27||7.8||23||August 07
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3305||+/-25||5.1||22||August 07
+
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||33.4||3.8||3||July 24
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_J23116 J23116]|| ''Eco''R I||''Spe'' I||Insert||52||+/-20||8.1||26||August 12
+
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||25||6.1||6||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||14.1||27||August 12
+
|[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||4080||+/-30||32.8||33||August 14
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_F1610 F1610]|| ''Eco''R I||''Xba'' I||Vector||3956||26||4.5||35||August 19
+
|[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||4086||+/-30||14.8||32||August 14
-
|- style="background: #E0FFFF; text-align: center;"
+
-
|[http://partsregistry.org/Part:BBa_K1450151 K145151]|| ''Eco''R I||''Spe'' I||Insert||306||19||25.2||36||August 19
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
|[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Xba'' I||Vector||13||2092||17.6||25||August 19
+
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
|[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||112||+/-30||3.9||28||August 14
|[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||112||+/-30||3.9||28||August 14
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||101||+/-30||4.0||29||August 14
|[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||101||+/-30||4.0||29||August 14
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||365||+/-20||6.9||30||August 14
 
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3113||+/-20||8.3||31||August 14
|[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3113||+/-20||8.3||31||August 14
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||4086||+/-30||14.8||32||August 14
+
|[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||71||+/-28||2.5||41||August 25
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||4080||+/-30||32.8||33||August 14
+
|[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_F1610 F1610]|| ''Xba''I||-||Vector||431||+/-5||25.9||40||August 25
-
|- style="background: #E0FFFF; text-align: center;" 
+
-
|[http://partsregistry.org/Part:BBa_C0062 C0062]|| ''Eco''R I||''Spe'' I||Insert||774||28||5.0||10||August 12
+
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||144||+/-20||0.9||18||August 22
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||144||+/-20||0.9||18||August 22
Line 277: Line 289:
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||38||August 25
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||38||August 25
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_F1610 F1610]|| ''Xba''I||-||Vector||431||+/-5||25.9||40||August 25
+
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||311||+/-28||3.6||45||August 27
-
|- style="background: #E0FFFF; text-align: center;" 
+
-
|[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||71||+/-28||2.5||41||August 25
+
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||144||+/-28||2.4||42||August 25
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||144||+/-28||2.4||42||August 25
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2056||24||27.7||43||August 25
+
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||365||+/-20||6.9||30||August 14
-
|- style="background: #E0FFFF; text-align: center;" 
+
-
|[http://partsregistry.org/Part:BBa_K145001 K145001]|| ''Eco''R I||''Spe'' I||Insert||2730||+/-21||24.3||24||August 22
+
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||432||+/-30||4.6||34||August 22
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||432||+/-30||4.6||34||August 22

Revision as of 14:50, 28 August 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

MiniPrep Products: Parts
Part Plasmid Description µl left ng/µl Box Date
B0015pSB1AK3Terminator+/- 10112.311July 24
B0015pSB1AK3Terminator+/- 20130.312July 24
B0015pSB1AK3Terminator+/- 43303.113July 29
B0015pSB1AK3Terminator+/- 20213.614July 29
B0015pSB1AK3Terminator+/- 48311.031July 29
B0015pSB1AK3Terminator+/- 30214.047July 29
B0015pSB1AK3Terminator+/- 40209.548July 29
B0015pSB1AK3Terminator+/- 4063.010July 23
B0032pSB1A2RBS(medium)+/- 551.056August 11
B0032pSB1A2RBS(medium)+/- 2047.257August 27
B0032pSB1A2RBS(medium)+/- 4027.058August 27
B0033pSB1AK3RBS+/- 5036.535August 27
B0033pSB1AK3RBS+/- 2338.441August 27
B0034pSB1A2RBS (efficiency 1)+/- 2073.85July 24
B0034pSB1A2RBS (efficiency 1)+/- 3095.16July 24
B0034pSB1A2RBS (efficiency 1)+/- 40104.07July 29
B0034pSB1AK3RBS+/- 325739August 27
B0034pSB1AK3RBS+/- 5029.640August 27
C0012pSB1A2LacI+/- 30133.121July 29
C0012pSB1A2LacI repressor+/- 40146.449July 29
C0012pSB1A2LacI repressor+/- 40203.150July 29
C0040pSB1A2TetR repressor+/- 20177.18July 24
C0040pSB1A2TetR repressor+/- 30161.79July 24
C0060pSB1A2lactonase production+/- 4884.254August 11
C0062pSB1A2LuxR repressor+/- 4876.255August 11
C0062pSB1AK3LuxR repressor+/- 2541.545August 27
C0062pSB1AK3LuxR repressor+/- 5031.749August 27
E0022pSB1A2eCFP+/- 4084.219July 23
E0022pSB1A2eCFP+/- 4086.420July 24
F1610pSB1AK3HSL Sender Device+/- 5057.617August 12
F1610pSB1AK3HSL Sender Device+/- 4053.118August 12
F1610pSB1AK3HSL Sender Device+/- 5041.422August 27
F1610pSB1AK3HSL Sender Device+/- 2031.533August 27
I12006pSB2K3434 cI repressible promoter +/- 4825.851August 08
I12006pSB2K3434 cI repressible promoter +/- 4852.752August 08
I712022BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
I712074BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.227July 29
I712074BBa_J52017 (Ap,Km)T7 Promotor+/- 4520.561August 27
I712074BBa_J52017 (Ap,Km)T7 Promotor+/- 4563.962August 27
J23022BBa_J23006 (Ap)3d key+ terminator+/- 40160.725July 29
J23022BBa_J23006 (Ap)3d key+ terminator+/- 46308.329July 29
J23032BBa_J23006 (Ap)3d lock+/- 35117.423July 29
J23032BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
J23032BBa_J23006 (Ap)3d lock+/- 48129.128July 29
J23100BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
J23100BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 46375.126July 29
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 4899.259August 27
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 4869.260August 27
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 4840430July 29
J23116BBa_J61002 (Ap)Constitutive promoter +/- 48328.853August 08
M30109pSB1AC3Light-sensor+/- 4050.41July 23
M30109pSB1AC3Light-sensor+/- 3054.32July 24
R0011pSB1A2LacI regulated promoter+/- 4564.043July 29
R0011pSB1A2LacI regulated promoter +/- 4069.844July 29
R0011pSB1AK3LacI regulated promoter+/- 5030.838August 27
R0040pSB1A2tetR repressible promoter+/- 4837.963August 11
R0062pSB1A2LuxR regulated promoter +/- 40104.137July 31
R0084pSB1A2OmpF promoter+/- 4032.015July 23
R0084pSB1A2OmpF promoter+/- 3060.916July 24


MiniPrep Products: Ligations
Part Plasmid Description µl left ng/µl Box Date
C0062+B0015pSB1AK3Ligation for Cell Death+/- 46124.81AAugust 08
E0022+B0015pSB1AK3Ligation for Output+/- 46151.85AAugust 08
I712074+J23032BBa_J23006 (Ap)Ligation for Filter+/- 4689.93AAugust 08
J23109+J23032BBa_J23006 (Ap)Ligation for Filter+/- 4694.44AAugust 08
J23116+B0032pSB1A2Ligation for Input+/- 40102.58AAugust 25
J23116+B0032pSB1A2Ligation for Input+/- 3596.22BAugust 25
K145001+pSB1A2pSB1A2T7 polymerase (no tag)+/- 4622.65BAugust 28
K145001+pSB1A2pSB1A2T7 polymerase (no tag)+/- 4628.36BAugust 28
K145001+pSB1A2pSB1A2T7 polymerase (no tag)+/- 4642.37BAugust 28
K145001+pSB1A2pSB1A2T7 polymerase (no tag)+/- 4638.18BAugust 28
K145015+pSB1A2pSB1A2GFP with LVA tag+/- 46152.72AAugust 08
K145151+pSB1A2pSB1A2ccdB coding region+/- 4657.89BAugust 28
K145151+pSB1A2pSB1A2ccdB coding region+/- 4672.11CAugust 28
K145151+pSB1A2pSB1A2ccdB coding region+/- 4618.52CAugust 28
K145151+pSB1A2pSB1A2ccdB coding region+/- 4623.33CAugust 28
R0040+B0032pSB1A2Ligation for output+/- 3064.49AAugust 25
R0040+B0032pSB1A2Ligation for Output+/- 3593.11BAugust 25
R0040+J23022BBa_J23006 (Ap)Ligation for Filter (old)+/- 3581.33BAugust 27
R0040+J23022BBa_J23006 (Ap)Ligation for Filter (old)+/- 3586.34BAugust 27
R0084+J23022+J23109+J23032BBa_J23006 (Ap)Ligation for Filter (old)+/- 20150.26AAugust 20
R0084+J23022+J23109+J23032BBa_J23006 (Ap)Ligation for Filter (old)+/- 2058.37AAugust 20


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
B0015 EcoR IXba IVector33031111.711July 31
B0015 EcoR IXba IVector33031013.617August 26
B0032 EcoR IXba IVector2092517.625August 19
B0033 EcoR IXba IVector20922010.939August 27
C0040 EcoR ISpe IInsert677187.12July 24
C0060 EcoR ISpe IInsert803285.420August 12
C0060 EcoR ISpe IInsert80698.215August 01
C0062 EcoR ISpe IInsert7741824.714August 01
C0062 EcoR ISpe IInsert774258.344August 27
C0062 EcoR ISpe IInsert774285.010August 12
E0022 EcoR ISpe IInsert779229.54July 24
F1610 EcoR IXba IVector3956264.535August 19
J23032 EcoR IXba IVector3090212.98July 30
J23032 EcoR IXba IVector3090451.39July 31
J23032 EcoR IXba IVector3308255.122August 07
J23100EcoR ISpe IInsert5238.12.61July 24
J23109 EcoR ISpe IInsert52214.25July 29
J23116 EcoR ISpe IInsert52+/-208.126August 12
K145001 (T7 poly) EcoR ISpe IInsert2732+/-814.127August 12
K145001 EcoR ISpe IInsert2730+/-2124.324August 22
K145151 EcoR ISpe IInsert3061925.236August 19
pSB1A2 EcoR ISpe IPlasmid2079918.216August 01
pSB1A2 EcoR ISpe IPlasmid20562427.743August 25
R0011 EcoR ISpe IInsert72208.512July 30
R0011 EcoR ISpe IInsert72207.513July 30
R0011 EcoR ISpe IInsert72252.121August 27
R0062 EcoR ISpe IInsert55277.823August 07
R0084 EcoR ISpe IInsert12533.43.83July 24
R0084 EcoR ISpe IInsert125256.16July 29
C0062+B0015 EcoR IXba IVector4080+/-3032.833August 14
E0022+B0015 EcoR IXba IVector4086+/-3014.832August 14
I712074+J23032 EcoR ISpe IInsert112+/-303.928August 14
J23109+J23032 EcoR ISpe IInsert101+/-304.029August 14
J23109+J23032 EcoR IXba IVector3113+/-208.331August 14
J23116+B0032 EcoR ISpe IInsert71+/-282.541August 25
R0011+F1610 XbaI-Vector431+/-525.940August 25
R0084+B0032 EcoR ISpe IInsert144+/-200.918August 22
R0084+B0032 EcoR ISpe IInsert144+/-302.919August 21
R0040+B0032 EcoR ISpe IInsert90+/-282.638August 25
R0040+J23022 EcoR ISpe IInsert311+/-283.645August 27
R0084+B0032 EcoR ISpe IInsert144+/-282.442August 25
R0084+J23022 EcoR ISpe IInsert365+/-206.930August 14
R0084+J23022+J23109+J23032 EcoR ISpe IInsert432+/-304.634August 22
R0084+J23022+J23109+J23032 EcoR ISpe IInsert432+/-303.737August 22

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer GFP-LVA10849020 µM1August 01
Primer GFP-LVA10859020 µM2August 01
dNTP mixATP, GTP, TTP, CTP7010 mM3August 01
Primer GFP-LVA1084500100 µM4August 01
Primer GFP-LVA1085500100 µM5August 01
Primer Test1074800100 µM6August 07
Primer Test1075780100 µM7August 07
Primer T7-UmuD1076560100 µM8August 07
Primer T7-UmuD1077477100 µM9August 07
Primer T7-UmuD1078467100 µM10August 07
Primer T7-UmuD107945100 µM11August 07
Primer Transduction1097627100 µM12August 07
Primer Transduction1098710100 µM13August 07
Primer Transduction1099608100 µM14August 07
Primer Anti-sense LuxI1100522100 µM15August 07
Primer Anti-sense LuxI1101237100 µM16August 07
Primer Test107410020 µM17August 07
Primer Test107510020 µM18August 07
Primer T7-UmuD107610020 µM19August 07
Primer T7-UmuD107710020 µM20August 07
Primer T7-UmuD107810020 µM21August 07
Primer T7-UmuD107910020 µM22August 07
Primer Transduction109710020 µM23August 07
Primer Transduction109810020 µM24August 07
Primer Transduction109910020 µM25August 07
Primer Anti-sense LuxI110010020 µM26August 07
Primer Anti-sense LuxI110110020 µM27August 07
dNTP mixATP, GTP, TTP, CTP902 mM28August 19
Primer ccdB1127100100 µM29August 19
Primer ccdB1128100100 µM30August 19
Primer K1451501129100100 µM31August 19
Primer K1451501130100100 µM32August 19
Primer ccdB11279020 µM33August 19
Primer ccdB112898020 µM34August 19
Primer K14515011299901 µM35August 19
Primer K14515011309901 µM36August 19
dNTP mixATP, GTP, TTP, CTP9902 µM37August 19
Retrieved from "http://2008.igem.org/Team:KULeuven/Freezer"