Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
m
(Parts stored in the fridge in the lab (4°C))
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|[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||32.5||9.5||4||July 24
|[http://partsregistry.org/Part:BBa_E0022 E0022]|| ''Eco''R I||''Spe'' I||Insert||779||32.5||9.5||4||July 24
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52||28||4.2||5||July 24
+
|[http://partsregistry.org/Part:BBa_J23109 J23109]|| ''Eco''R I||''Spe'' I||Insert||52||28||4.2||5||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||28||6.1||6||July 24
+
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||28||6.1||6||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||62||28||3.4||7||July 24
+
|[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||62||28||3.4||7||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||20||20.9||8||July 30
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3090||10||12.9||9||July 30
|}
|}
== Glycerol stock of cells (-80°C) ==
== Glycerol stock of cells (-80°C) ==

Revision as of 15:07, 30 July 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 4050.41July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 3054.32July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.85July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.16July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.07July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 25177.18July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.79July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.010July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.311July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.312July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 45303.113July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30213.614July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.015July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.916July 24
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS (efficiency 0.3)+/- 4031.217July 23
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS (efficiency 0.3)+/- 10114.818July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4084.219July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4086.420July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.121July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006(Ap)3d lock+/- 40117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006(Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006(Ap)3d lock+/- 48129.128July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006(Ap)3d key+ terminator+/- 40160.725July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006(Ap)3d key+ terminator+/- 48308.329July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]BBa_J52017(Ap,Km)Terminator+/- 48311.031July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002(Ap)Constitutive Promotor+/- 46375.126July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002(Ap)constitutive Pr+/- 4840430July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017(Ap,Km)T7 Promotor+/- 42.5208.227July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 40104.022July 29


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.61July 24
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert67731.47.12July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.83July 24
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert77932.59.54July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52284.25July 29
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125286.16July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert62283.47July 29
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33032020.98July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector30901012.99July 30

Glycerol stock of cells (-80°C)