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Team:KULeuven/Freezer
From 2008.igem.org
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Parts stored in the fridge in the lab (4°C)
| Plasmids: MiniPrep Products | ||||||
|---|---|---|---|---|---|---|
| Part | Plasmid | Description | µl left | ng/µl | Box | Date |
| M30109 | pSB1AC3 | Light-sensor | +/- 40 | 50.4 | 1 | July 23 |
| M30109 | pSB1AC3 | Light-sensor | +/- 30 | 54.3 | 2 | July 24 |
| J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 60 | 345.0 | 3 | July 24 |
| J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 30 | 176.1 | 4 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 5 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 6 | July 24 |
| B0034 | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 7 | July 29 |
| C0040 | pSB1A2 | TetR repressor | +/- 25 | 177.1 | 8 | July 24 |
| C0040 | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 9 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 40 | 63.0 | 10 | July 23 |
| B0015 | pSB1AK3 | Terminator | +/- 10 | 112.3 | 11 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 20 | 130.3 | 12 | July 24 |
| B0015 | pSB1AK3 | Terminator | +/- 45 | 303.1 | 13 | July 29 |
| B0015 | pSB1AK3 | Terminator | +/- 30 | 213.6 | 14 | July 29 |
| B0015 | pSB1AK3 | Terminator | +/- 48 | 311.0 | 31 | July 29 |
| B0015 | pSB1AK3 | Terminator | +/- 40 | 214.0 | 47 | July 29 |
| B0015 | pSB1AK3 | Terminator | +/- 40 | 209.5 | 48 | July 29 |
| R0084 | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 15 | July 23 |
| R0084 | pSB1A2 | OmpF promoter | +/- 30 | 60.9 | 16 | July 24 |
| B0032 | pSB1A2 | RBS (efficiency 0.3) | +/- 10 | 114.8 | 18 | July 24 |
| E0022 | pSB1A2 | eCFP | +/- 40 | 84.2 | 19 | July 23 |
| E0022 | pSB1A2 | eCFP | +/- 40 | 86.4 | 20 | July 24 |
| C0012 | pSB1A2 | LacI | +/- 30 | 133.1 | 21 | July 29 |
| C0012 | pSB1A2 | LacI repressor | +/- 40 | 146.4 | 49 | July 29 |
| C0012 | pSB1A2 | LacI repressor | +/- 40 | 203.1 | 50 | July 29 |
| J23032 | BBa_J23006 (Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
| J23032 | BBa_J23006 (Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
| J23032 | BBa_J23006 (Ap) | 3d lock | +/- 48 | 129.1 | 28 | July 29 |
| J23022 | BBa_J23006 (Ap) | 3d key+ terminator | +/- 40 | 160.7 | 25 | July 29 |
| J23022 | BBa_J23006 (Ap) | 3d key+ terminator | +/- 46 | 308.3 | 29 | July 29 |
| J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 46 | 375.1 | 26 | July 29 |
| J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 404 | 30 | July 29 |
| I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 27 | July 29 |
| F1610 | pSB1AK3 | RBS + LuxI + Terminator | +/- 30 | 104.0 | 22 | July 29 |
| F1610 | pSB1AK3 | RBS + LuxI + Terminator | +/- 40 | 170.8 | 45 | July 29 |
| F1610 | pSB1AK3 | RBS + LuxI + Terminator | +/- 40 | 159.0 | 46 | July 29 |
| I712022 | BBa_J52017 (Km, Ap) | T7 polymerase | +/- 40 | 394.1 | 35 | July 31 |
| R0062 | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 37 | July 31 |
| R0011 | pSB1A2 | LacI regulated promoter | +/- 45 | 64.0 | 43 | July 29 |
| R0011 | pSB1A2 | LacI regulated promoter | +/- 40 | 69.8 | 44 | July 29 |
| P1010+B0015 | pSB1AK3 | Ligation for Cell Death | +/- 48 | 119.1 | 38 | August 06 |
| C0040+B0015 | pSB1AK3 | Ligation for TetR generator | +/- 48 | 131.0 | 39 | August 06 |
| C0040+B0015 | pSB1AK3 | Ligation for TetR generator | +/- 48 | 133.2 | 40 | August 06 |
| C0060+B0015 | pSB1AK3 | Ligation for Reset | +/- 48 | 118.5 | 41 | August 06 |
| J23100+B0032 | pSB1A2 | Ligation for Cell Death | +/- 46 | 41.0 | 42 | August 06 |
| Restriction Digests | ||||||||
|---|---|---|---|---|---|---|---|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
| J23100 | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 1 | July 24 |
| C0040 | EcoR I | Spe I | Insert | 677 | 25 | 7.1 | 2 | July 24 |
| R0084 | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 3 | July 24 |
| E0022 | EcoR I | Spe I | Insert | 779 | 22 | 9.5 | 4 | July 24 |
| J23109 | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 5 | July 29 |
| R0084 | EcoR I | Spe I | Insert | 125 | 28 | 6.1 | 6 | July 29 |
| I712074 | EcoR I | Spe I | Insert | 62 | 23 | 3.4 | 7 | July 29 |
| J23032 | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 8 | July 30 |
| J23032 | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 9 | July 31 |
| J23032 | EcoR I | Xba I | Vector | 3308 | 25 | 5.1 | 22 | August 07 |
| B0032 | EcoR I | Xba I | Vector | 2077 | 20 | 4.0 | 10 | July 31 |
| B0015 | EcoR I | Xba I | Vector | 3303 | 31 | 11.7 | 11 | July 31 |
| B0015 | EcoR I | Xba I | Vector | 3303 | 25 | 13.4 | 20 | August 07 |
| R0011 | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 12 | July 30 |
| R0011 | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 13 | July 30 |
| C0062 | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 14 | August 01 |
| C0060 | EcoR I | Spe I | Insert | 806 | 15 | 8.2 | 15 | August 01 |
| pSB1A2 | EcoR I | Spe I | Plasmid | 2079 | 17 | 18.2 | 16 | August 01 |
| P1010 | EcoR I | Spe I | Insert | 682 | 10 | 4.9 | 17 | August 01 |
| K145015 (GFP-LVA) | EcoR I | Spe I | Insert | 625 | 13 | 6.3 | 18 | August 05 |
| C0012 | EcoR I | Spe I | Insert | 1145 | 25 | 5.6 | 19 | August 05 |
| J23022 | EcoR I | Xba I | Vector | 3498 | 25 | 13.2 | 21 | August 07 |
Things stored in freezer in lab (-20°C)
| Miscellaneous | |||||
|---|---|---|---|---|---|
| Description | Name | µl left | Concentration | Box | Date |
| Primer | 1084 | 990 | 20 µM | 1 | August 01 |
| Primer | 1085 | 990 | 20 µM | 2 | August 01 |
| dNTP mix | ATP, GTP, TTP, CTP | 990 | 10 mM | 3 | August 01 |
| Primer | 1084 | 500 | 100 µM | 4 | August 01 |
| Primer | 1085 | 500 | 100 µM | 5 | August 01 |
