Team:KULeuven/Freezer

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dock/undock dropdown

Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part	Plasmid	Description	µl left	ng/µl	Box	Date
M30109	pSB1AC3	Light-sensor	+/- 40	50.4	1	July 23
M30109	pSB1AC3	Light-sensor	+/- 30	54.3	2	July 24
J23100	BBa_J61002 (Ap)	Constitutive promoter	+/- 60	345.0	3	July 24
J23100	BBa_J61002 (Ap)	Constitutive promoter	+/- 30	176.1	4	July 24
B0034	pSB1A2	RBS (efficiency 1)	+/- 20	73.8	5	July 24
B0034	pSB1A2	RBS (efficiency 1)	+/- 30	95.1	6	July 24
B0034	pSB1A2	RBS (efficiency 1)	+/- 40	104.0	7	July 29
C0040	pSB1A2	TetR repressor	+/- 25	177.1	8	July 24
C0040	pSB1A2	TetR repressor	+/- 30	161.7	9	July 24
B0015	pSB1AK3	Terminator	+/- 40	63.0	10	July 23
B0015	pSB1AK3	Terminator	+/- 10	112.3	11	July 24
B0015	pSB1AK3	Terminator	+/- 20	130.3	12	July 24
B0015	pSB1AK3	Terminator	+/- 45	303.1	13	July 29
B0015	pSB1AK3	Terminator	+/- 30	213.6	14	July 29
B0015	pSB1AK3	Terminator	+/- 48	311.0	31	July 29
B0015	pSB1AK3	Terminator	+/- 40	214.0	47	July 29
B0015	pSB1AK3	Terminator	+/- 40	209.5	48	July 29
R0084	pSB1A2	OmpF promoter	+/- 40	32.0	15	July 23
R0084	pSB1A2	OmpF promoter	+/- 30	60.9	16	July 24
B0032	pSB1A2	RBS (efficiency 0.3)	+/- 10	114.8	18	July 24
E0022	pSB1A2	eCFP	+/- 40	84.2	19	July 23
E0022	pSB1A2	eCFP	+/- 40	86.4	20	July 24
C0012	pSB1A2	LacI	+/- 30	133.1	21	July 29
C0012	pSB1A2	LacI repressor	+/- 40	146.4	49	July 29
C0012	pSB1A2	LacI repressor	+/- 40	203.1	50	July 29
J23032	BBa_J23006 (Ap)	3d lock	+/- 35	117.4	23	July 29
J23032	BBa_J23006 (Ap)	3d lock	+/- 39.5	144.8	24	July 29
J23032	BBa_J23006 (Ap)	3d lock	+/- 48	129.1	28	July 29
J23022	BBa_J23006 (Ap)	3d key+ terminator	+/- 40	160.7	25	July 29
J23022	BBa_J23006 (Ap)	3d key+ terminator	+/- 46	308.3	29	July 29
J23109	BBa_J61002 (Ap)	Constitutive Promoter	+/- 46	375.1	26	July 29
J23109	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	404	30	July 29
I712074	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 42.5	208.2	27	July 29
F1610	pSB1AK3	RBS + LuxI + Terminator	+/- 30	104.0	22	July 29
F1610	pSB1AK3	RBS + LuxI + Terminator	+/- 35	170.8	45	July 29
I712022	BBa_J52017 (Km, Ap)	T7 polymerase	+/- 40	394.1	35	July 31
R0062	pSB1A2	LuxR regulated promoter	+/- 40	104.1	37	July 31
R0011	pSB1A2	LacI regulated promoter	+/- 45	64.0	43	July 29
R0011	pSB1A2	LacI regulated promoter	+/- 40	69.8	44	July 29
I12006	pSB2K3	434 cI repressible promoter	+/- 48	25.8	51	August 08
I12006	pSB2K3	434 cI repressible promoter	+/- 48	52.7	52	August 08
J23116	BBa_J61002 (Ap)	Constitutive promoter	+/- 48	328.8	53	August 08
C0060	pSB1A2	lactonase production	+/- 48	84.2	60	August 11
C0062	pSB1A2	LuxR repressor	+/- 48	76.2	61	August 11
B0032	pSB1A2	RBS(medium)	+/- 48	51.0	62	August 11
R0040	pSB1A2	tetR repressible promoter	+/- 48	37.9	63	August 11
P1010+B0015	pSB1AK3	Ligation for Cell Death	+/- 48	119.1	38	August 06
C0040+B0015	pSB1AK3	Ligation for TetR generator	+/- 48	131.0	39	August 06
C0040+B0015	pSB1AK3	Ligation for TetR generator	+/- 48	133.2	40	August 06
C0060+B0015	pSB1AK3	Ligation for Reset	+/- 48	118.5	41	August 06
J23100+B0032	pSB1A2	Ligation for Cell Death	+/- 46	41.0	42	August 06
C0062+B0015	pSB1AK3	Ligation for Cell Death	+/- 46	124.8	54	August 08
K145015+pSB1A2	pSB1A2	GFP with LVA tag	+/- 46	152.7	55	August 08
K145015+B0015	pSB1A2	Ligation for Output	+/- 46	17.5	56	August 08
I712074+J23032	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	89.9	57	August 08
J23109+J23032	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	94.4	58	August 08
E0022+B0015	pSB1AK3	Ligation for Output	+/- 46	151.8	59	August 08

Restriction Digests
Part	Enzyme 1	Enzyme 2	vector/insert	length (bp)	µl left	ng/µl	Box	Date
J23100	EcoR I	Spe I	Insert	52	38.1	2.6	1	July 24
C0040	EcoR I	Spe I	Insert	677	25	7.1	2	July 24
R0084	EcoR I	Spe I	Insert	125	33.4	3.8	3	July 24
E0022	EcoR I	Spe I	Insert	779	22	9.5	4	July 24
J23109	EcoR I	Spe I	Insert	52	21	4.2	5	July 29
R0084	EcoR I	Spe I	Insert	125	25	6.1	6	July 29
I712074	EcoR I	Spe I	Insert	62	23	3.4	7	July 29
J23032	EcoR I	Xba I	Vector	3090	2	12.9	8	July 30
J23032	EcoR I	Xba I	Vector	3090	45	1.3	9	July 31
J23032	EcoR I	Xba I	Vector	3308	25	5.1	22	August 07
B0015	EcoR I	Xba I	Vector	3303	31	11.7	11	July 31
B0015	EcoR I	Xba I	Vector	3303	21	13.4	20	August 07
R0011	EcoR I	Spe I	Insert	72	20	8.5	12	July 30
R0011	EcoR I	Spe I	Insert	72	20	7.5	13	July 30
C0062	EcoR I	Spe I	Insert	774	18	24.7	14	August 01
C0060	EcoR I	Spe I	Insert	806	15	8.2	15	August 01
pSB1A2	EcoR I	Spe I	Plasmid	2079	17	18.2	16	August 01
P1010	EcoR I	Spe I	Insert	682	10	4.9	17	August 01
K145015 (GFP-LVA)	EcoR I	Spe I	Insert	625	13	6.3	18	August 05
C0012	EcoR I	Spe I	Insert	1145	12	5.6	19	August 05
J23022	EcoR I	Xba I	Vector	3498	20	15.2	21	August 07
R0062	EcoR I	Spe I	Insert	55	27	7.8	23	August 07
J23032	EcoR I	Xba I	Vector	3305	+/-25	5.1	22	August 07

Things stored in freezer in lab (-20°C)

Miscellaneous
Description	Name	µl left	Concentration	Box	Date
Primer GFP-LVA	1084	90	20 µM	1	August 01
Primer GFP-LVA	1085	90	20 µM	2	August 01
dNTP mix	ATP, GTP, TTP, CTP	70	10 mM	3	August 01
Primer GFP-LVA	1084	500	100 µM	4	August 01
Primer GFP-LVA	1085	500	100 µM	5	August 01
Primer Test	1074	800	100 µM	6	August 07
Primer Test	1075	780	100 µM	7	August 07
Primer T7-UmuD	1076	560	100 µM	8	August 07
Primer T7-UmuD	1077	477	100 µM	9	August 07
Primer T7-UmuD	1078	467	100 µM	10	August 07
Primer T7-UmuD	1079	45	100 µM	11	August 07
Primer Transduction	1097	627	100 µM	12	August 07
Primer Transduction	1098	710	100 µM	13	August 07
Primer Transduction	1099	608	100 µM	14	August 07
Primer Anti-sense LuxI	1100	522	100 µM	15	August 07
Primer Anti-sense LuxI	1101	237	100 µM	16	August 07
Primer Test	1074	100	20 µM	17	August 07
Primer Test	1075	100	20 µM	18	August 07
Primer T7-UmuD	1076	100	20 µM	19	August 07
Primer T7-UmuD	1077	100	20 µM	20	August 07
Primer T7-UmuD	1078	100	20 µM	21	August 07
Primer T7-UmuD	1079	100	20 µM	22	August 07
Primer Transduction	1097	100	20 µM	23	August 07
Primer Transduction	1098	100	20 µM	24	August 07
Primer Transduction	1099	100	20 µM	25	August 07
Primer Anti-sense LuxI	1100	100	20 µM	26	August 07
Primer Anti-sense LuxI	1101	100	20 µM	27	August 07
dNTP mix	ATP, GTP, TTP, CTP	90	2 mM	28	August 07

Ligations: step 1

Inputs
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
J23116+B0032	-	-	-	-	-
C0040+B0015	ok	ok	-	-	-
R0040+E0240	-	-	-	-	-
Output
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
R0084+B0032	ok	ok	ok	-	-
E0022+B0015	ok	ok	ok	ok	ok
K145015+B0015	pcr	ok	ok	ok	-
E0040+B0015	-	-	-	-	-
Memory
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
R0053+P0152	-	-	-	-	-
R1052+P0353	-	-	-	-	-
R0084+B0033	-	-	-	-	-
C0056+B0015	-	-	-	-	-
antisense?	-	-	-	-	-
Filter
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
R0084+J23022	ok	ok	ok	-	-
J230109+J23032	ok	ok	ok	ok	-
K145014+B0015	-	-	-	-	-
K145001+B0015	-	-	-	-	-
I7122074+J23032	ok	ok	ok	ok	-
IK0145015+B0015	pcr	ok	ok	ok	-
E0040+B0015	-	-	-	-	-
Inverter
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
I712074+J23032	ok	ok	ok	ok	-
C0012+B0015	ok	ok	ok	-	-
R0011+F1610	ok	-	-	-	-
R0011+E0240	-	-	-	-	-
Reset
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
I712074+J023022	ok	ok	ok	ok	-
C0012+B0015	-	ok	ok	ok	-
Cell Death
Name	Miniprep	Digest	Ligation	Colonies	Confirmed
J23100+B0032	ok	ok	ok	ok	-
C0062+B0015	-	ok	ok	ok	ok
R0062+B0032	ok	ok	ok	-	-
P1010+B0015	-	ok	ok	ok	-
R0062+E0240	-	-	-	-	-