Team:KULeuven/Ligation

From 2008.igem.org

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{{:Team:KULeuven/Tools/Styling}}
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{{:Team:KULeuven/Tools/Scripting}}
{{:Team:KULeuven/Tools/Header}}
{{:Team:KULeuven/Tools/Header}}
-
=Ligations: step 1=
+
== New Parts ==
-
{|
+
 
-
|- style="background: #003E81;color:white;width=100%"
+
{| style="background:#99CCFF; color:black; width:85%; text-align:center;" border="1" cellpadding="2" cellspacing="0"
-
! colspan="6" style="background: #003E81" | Inputs
+
! width=75|BioBrick
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|- style="background: #99CCFF; text-align: center; width=100%"
+
! width=645|Description
-
|width=200| '''Name'''
+
! width=70|Status
-
|width=160| '''Miniprep'''
+
! width=70|Works?
-
|width=150| '''Digest'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Ligation'''
+
| [http://partsregistry.org/Part:BBa_K145001 K145001] || T7 DNA polymerase (no tag)|| [[Image:correct.JPG|center|30px]] ||  
-
|width=150| '''Colonies'''
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Confirmed'''
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| [http://partsregistry.org/Part:BBa_K145013 K145013] || antisense LuxI|| [[Image:correct.JPG|center|30px]] ||  
-
|- style="background: #E0FFFF; text-align: center;"  
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|J23116+B0032||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145014 K145014] || T7 DNA polymerase with UmuD tag|| [[Image:correct.JPG|center|30px]] ||  
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|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|C0040+B0015||ok||ok||-||ok||-
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| [http://partsregistry.org/Part:BBa_K145015 K145015] || GFP with LVA tag|| [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|R0040+E0240||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145150 K145150] || hybrid promoter|| [[Image:correct.JPG|center|30px]] ||
-
|- style="background: #003E81;color:white;width=100%"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
! colspan="6" style="background: #003E81" | Output
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| [http://partsregistry.org/Part:BBa_K145151 K145151] || ccdB coding region || [[Image:correct.JPG|center|30px]] ||  
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
|}
-
|width=200| '''Name'''
+
 
-
|width=160| '''Miniprep'''
+
==New Devices ==
-
|width=150| '''Digest'''
+
 
-
|width=150| '''Ligation'''
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{| style="background:#99CCFF; color:black; width:85%; text-align:center;" border="1" cellpadding="2" cellspacing="0"
-
|width=150| '''Colonies'''
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! width=100 |'''BioBrick'''  
-
|width=150| '''Confirmed'''
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! width=450 | '''Components'''
-
|- style="background: #E0FFFF; text-align: center;"
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! width=200 | '''Description'''
-
| R0084+B0032||ok||ok||ok||-||-
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! width=100 | '''Status*'''
-
|- style="background: #E0FFFF; text-align: center;"
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! width=100 | '''Works?'''
-
| E0022+B0015||ok||ok||ok||ok||ok
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
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| [http://partsregistry.org/Part:BBa_K145201 K145201] || [[Image:K145201.JPG|center]] || Input || [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
| K145015+B0015||pcr||ok||ok||ok||-
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145205 K145205] || [[Image:K145205.JPG|center]] || Output (LVA tag)|| [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
| E0040+B0015||-||-||-||-||-
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #003E81;color:white;width=100%"
+
| [http://partsregistry.org/Part:BBa_K145252 K145252] || [[Image:K145252.JPG|center]] || Output (no tag) || [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
! colspan="6" style="background: #003E81" | Memory
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
| [http://partsregistry.org/Part:BBa_K145280 K145280] || [[Image:K145280.JPG|center]]|| Input-Output: test || [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
|width=200| '''Name'''
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|- style="background:#ffffff; color:black; text-align:center; height:50px"
-
|width=160| '''Miniprep'''
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| [http://partsregistry.org/Part:BBa_K145279 K145279] || [[Image:K145279.JPG|center]] || Input-Output: test || [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
|width=150| '''Digest'''
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|width=150| '''Ligation'''
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| [http://partsregistry.org/Part:BBa_K145258 K145258] || [[Image:K145258.JPG|center]] || Memory - part 1 || ||  
-
|width=150| '''Colonies'''
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|width=150| '''Confirmed'''
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| [http://partsregistry.org/Part:BBa_K145259 K145259] || [[Image:K145259.JPG|center]] || Memory - part 2 || ||  
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
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| R0053+P0152 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145210 K145210] || [[Image:K145210.JPG|center]] || Memory || ||  
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| R1052+P0353 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145211 K145211] || [[Image:K145211.JPG|center]] || Memory - part 3 || ||  
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| R0084+B0033 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145215 K145215] || [[Image:K145215.JPG|center|150px]]  || Filter - part 1 || [[Image:correct.JPG|center|30px]] ||  
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|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| C0056+B0015 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145278 K145278] || [[Image:K145278.JPG|center]]  || Filter (old) - part 2 || ||
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|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black; text-align:center; height:50px"
-
| R1052+K145013 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145277 K145277] || [[Image:K145277.JPG|center]]  || Filter (new) - part 2 ||  ||
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|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
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| K145013+B0015 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145275 K145275] || [[Image:K145275.JPG|center]]  || Filter (old) || ||  
-
|- style="background: #003E81;color:white;width=100%"
+
|- style="background:#ffffff; color:black; text-align:center; height:50px"
-
! colspan="6" style="background: #003E81" | Filter
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| [http://partsregistry.org/Part:BBa_K145276 K145276] || [[Image:K145276.JPG|center]]  || Filter (new) || ||  
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|- style="background: #99CCFF; text-align: center; width=100%"
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|width=200| '''Name'''
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| [http://partsregistry.org/Part:BBa_K145253 K145253] || [[Image:K145253.JPG|center]]  || Invertimer (old) - part 1 || PCR ||  
-
|width=160| '''Miniprep'''
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|width=150| '''Digest'''
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| [http://partsregistry.org/Part:BBa_K145255 K145255] || [[Image:K145255.JPG|center]]  || Invertimer (new) - part 1 || ||  
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|width=150| '''Ligation'''
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|width=150| '''Colonies'''
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| [http://partsregistry.org/Part:BBa_K145254 K145254] || [[Image:K145254.JPG|center]]  || Invertimer - part 2 || [[Image:correct.JPG|center|30px]] ||  
-
|width=150| '''Confirmed'''
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
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| [http://partsregistry.org/Part:BBa_K145220 K145220] || [[Image:K145220.JPG|center|300px]]  || Invertimer (new) || ||  
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| R0084+J23022 ||ok||ok||ok||-||-
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|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145104 K145104] || [[Image:K145104.JPG|center]]  || Invertimer (old) || PCR ||  
-
| J230109+J23032 ||ok||ok||ok||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145251 K145251] || [[Image:K145251.JPG|center]]  || Reset (old) || PCR ||  
-
| K145014+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black; text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145225 K145225] || [[Image:K145225.JPG|center]]  || Reset (new) || ||  
-
| K145001+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145256 K145256] || [[Image:K145256.JPG|center]]  || Cell Death - part 1 || ||  
-
| I7122074+J23032 ||ok||ok||ok||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145257 K145257] || [[Image:K145257.JPG|center]]  || Cell Death - part 2 || [[Image:correct.JPG|center|30px]] ||
-
| K145015+B0015 ||pcr||ok||ok||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145230 K145230] || [[Image:K145230.JPG|center]]  || Cell Death || ||  
-
| E0040+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|- style="background: #003E81;color:white;width=100%"
+
| [http://partsregistry.org/Part:BBa_K145273 K145273] || [[Image:K145273.JPG|center]]  || test K145150 - part 2 || [[Image:correct.JPG|center|30px]] ||  
-
! colspan="6" style="background: #003E81" | Inverter
+
|- style="background:#ffffff; color:black; text-align:center; height:50px"
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
| [http://partsregistry.org/Part:BBa_K145270 K145270] || [[Image:K145270.JPG|center]]  || test K145150 - part 3 || ||
-
|width=200| '''Name'''
+
|- style="background:#ffffff; color:black; text-align:center; height:50px"
-
|width=160| '''Miniprep'''
+
| [http://partsregistry.org/Part:BBa_K145269 K145269] || [[Image:K145269.JPG|center]]  || test K145150 - part 4 || [[Image:correct.JPG|center|30px]] ||  
-
|width=150| '''Digest'''
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
|width=150| '''Ligation'''
+
| [http://partsregistry.org/Part:BBa_K145268 K145268] || [[Image:K145268.JPG|center]]  || test K145150 - part 5 || ||
-
|width=150| '''Colonies'''
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|- style="background:#ffffff; color:black; text-align:center; height:45px"
-
|width=150| '''Confirmed'''
+
| [http://partsregistry.org/Part:BBa_K145267 K145267] || [[Image:K145267.JPG|center]]  || test K145150 - part 6 || ||  
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| I712074+J23032||ok||ok||ok||ok||-
+
| [http://partsregistry.org/Part:BBa_K145266 K145266] || [[Image:K145266.JPG|center|450px]]  || test K145150|| ||  
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| C0012+B0015||ok||ok||ok||-||-
+
| [http://partsregistry.org/Part:BBa_K145271 K145271] || [[Image:K145271.JPG|center]]  || test filter (new) - part 3 || ||  
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| R0011+F1610||ok||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145272 K145272] || [[Image:K145272.JPG|center]]  || test filter (old) - part 3 || PCR ||  
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
| R0011+E0240||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145265 K145265] || [[Image:K145265.JPG|center|410px]]  || test filter (old) || ||
-
|- style="background: #003E81;color:white;width=100%"
+
|- style="background:#ffffff; color:black;  text-align:center; height:50px"
-
! colspan="6" style="background: #003E81" | Reset
+
| [http://partsregistry.org/Part:BBa_K145264 K145264] || [[Image:K145264.JPG|center|410px]]  || test filter (new) || ||
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
-
|width=200| '''Name'''
+
-
|width=160| '''Miniprep'''
+
-
|width=150| '''Digest'''
+
-
|width=150| '''Ligation'''
+
-
|width=150| '''Colonies'''
+
-
|width=150| '''Confirmed'''
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| I712074+J023022||ok||ok||ok||ok||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| C0060+B0015||-||ok||ok||ok||-
+
-
|- style="background: #003E81;color:white;width=100%"
+
-
! colspan="6" style="background: #003E81" | Cell Death
+
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
-
|width=200| '''Name'''
+
-
|width=160| '''Miniprep'''
+
-
|width=150| '''Digest'''
+
-
|width=150| '''Ligation'''
+
-
|width=150| '''Colonies'''
+
-
|width=150| '''Confirmed'''
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| J23100+B0032||ok||ok||ok||ok||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| C0062+B0015||-||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0062+B0032||ok||ok||ok||-||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| P1010+B0015||-||ok||ok||ok||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0062+E0240||-||-||-||-||-
+
|}
|}

Latest revision as of 22:41, 29 October 2008

  dock/undock dropdown  

New Parts

BioBrick Description Status Works?
[http://partsregistry.org/Part:BBa_K145001 K145001] T7 DNA polymerase (no tag)
Correct.JPG
[http://partsregistry.org/Part:BBa_K145013 K145013] antisense LuxI
Correct.JPG
[http://partsregistry.org/Part:BBa_K145014 K145014] T7 DNA polymerase with UmuD tag
Correct.JPG
[http://partsregistry.org/Part:BBa_K145015 K145015] GFP with LVA tag
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145150 K145150] hybrid promoter
Correct.JPG
[http://partsregistry.org/Part:BBa_K145151 K145151] ccdB coding region
Correct.JPG

New Devices

BioBrick Components Description Status* Works?
[http://partsregistry.org/Part:BBa_K145201 K145201]
K145201.JPG
 Input
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145205 K145205]
K145205.JPG
 Output (LVA tag)
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145252 K145252]
K145252.JPG
 Output (no tag)
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145280 K145280]
K145280.JPG
 Input-Output: test
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145279 K145279]
K145279.JPG
 Input-Output: test
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145258 K145258]
K145258.JPG
 Memory - part 1
[http://partsregistry.org/Part:BBa_K145259 K145259]
K145259.JPG
 Memory - part 2
[http://partsregistry.org/Part:BBa_K145210 K145210]
K145210.JPG
 Memory
[http://partsregistry.org/Part:BBa_K145211 K145211]
K145211.JPG
 Memory - part 3
[http://partsregistry.org/Part:BBa_K145215 K145215]
K145215.JPG
 Filter - part 1
Correct.JPG
[http://partsregistry.org/Part:BBa_K145278 K145278]
K145278.JPG
 Filter (old) - part 2
[http://partsregistry.org/Part:BBa_K145277 K145277]
K145277.JPG
 Filter (new) - part 2
[http://partsregistry.org/Part:BBa_K145275 K145275]
K145275.JPG
 Filter (old)
[http://partsregistry.org/Part:BBa_K145276 K145276]
K145276.JPG
 Filter (new)
[http://partsregistry.org/Part:BBa_K145253 K145253]
K145253.JPG
 Invertimer (old) - part 1 PCR
[http://partsregistry.org/Part:BBa_K145255 K145255]
K145255.JPG
 Invertimer (new) - part 1
[http://partsregistry.org/Part:BBa_K145254 K145254]
K145254.JPG
 Invertimer - part 2
Correct.JPG
[http://partsregistry.org/Part:BBa_K145220 K145220]
K145220.JPG
 Invertimer (new)
[http://partsregistry.org/Part:BBa_K145104 K145104]
K145104.JPG
 Invertimer (old) PCR
[http://partsregistry.org/Part:BBa_K145251 K145251]
K145251.JPG
 Reset (old) PCR
[http://partsregistry.org/Part:BBa_K145225 K145225]
K145225.JPG
 Reset (new)
[http://partsregistry.org/Part:BBa_K145256 K145256]
K145256.JPG
 Cell Death - part 1
[http://partsregistry.org/Part:BBa_K145257 K145257]
K145257.JPG
 Cell Death - part 2
Correct.JPG
[http://partsregistry.org/Part:BBa_K145230 K145230]
K145230.JPG
 Cell Death
[http://partsregistry.org/Part:BBa_K145273 K145273]
K145273.JPG
 test K145150 - part 2
Correct.JPG
[http://partsregistry.org/Part:BBa_K145270 K145270]
K145270.JPG
 test K145150 - part 3
[http://partsregistry.org/Part:BBa_K145269 K145269]
K145269.JPG
 test K145150 - part 4
Correct.JPG
[http://partsregistry.org/Part:BBa_K145268 K145268]
K145268.JPG
 test K145150 - part 5
[http://partsregistry.org/Part:BBa_K145267 K145267]
K145267.JPG
 test K145150 - part 6
[http://partsregistry.org/Part:BBa_K145266 K145266]
K145266.JPG
 test K145150
[http://partsregistry.org/Part:BBa_K145271 K145271]
K145271.JPG
 test filter (new) - part 3
[http://partsregistry.org/Part:BBa_K145272 K145272]
K145272.JPG
 test filter (old) - part 3 PCR
[http://partsregistry.org/Part:BBa_K145265 K145265]
K145265.JPG
 test filter (old)
[http://partsregistry.org/Part:BBa_K145264 K145264]
K145264.JPG
 test filter (new)
Retrieved from "http://2008.igem.org/Team:KULeuven/Ligation"