Team:KULeuven/Parts

From 2008.igem.org

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== Submitted Parts ==
 
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{|
 
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|- style="background: #003E81;color:white;width:100%;"
 
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! colspan="6" style="background #003E81" | Submitted parts: Listing
 
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|- style="background: #99CCFF; text-align: center;"
 
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|width=90| '''Part number'''
 
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|width=90| '''Replaced by'''
 
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|wodth=470| '''Description'''
 
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|width=90| '''Type'''
 
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|width=110| '''Component'''
 
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|width=90| '''Status'''
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145000'''
 
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| '''K145100'''
 
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| eCFP with LVA tag under OmpR control
 
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| Part
 
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| Output
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145001'''
 
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| /
 
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| T7 RNA polymerase
 
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| Part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145002'''
 
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| /
 
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| T7 RNA Polymerase under OmpF control
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145003'''
 
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| '''K145102'''
 
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| RiboKey 3d under control of T7 RNA polymerase promotor
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145004'''
 
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| '''K145104'''
 
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| T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac.
 
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| Composite part
 
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| Inverter
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145005'''
 
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| /
 
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| cI under control of a dual promotor and a RiboLock.
 
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| Composite part
 
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| Pulse generator
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145006'''
 
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| '''K145106'''
 
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| Lactonase under PRM control
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145007'''
 
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| '''K145107'''
 
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| Pulse Generator
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145008'''
 
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| '''K145108'''
 
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| Constitutive production of LuxR
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145009'''
 
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| '''K145109'''
 
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| ccdB cell death gene under control of an activating Lux PR
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145010'''
 
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| '''K145110'''
 
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| Killing switch
 
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| Composite part
 
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| ''(nowhere)''
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145011'''
 
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| /
 
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| Light sensor to provide dummy input for the system, copy of '''BBa_M30109'''
 
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| Composite part
 
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| Input
 
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| Deleted
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145012'''
 
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| /
 
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| N-terminal degradation tag
 
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| Part
 
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| Filter
 
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| Not used
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145013'''
 
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| /
 
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| antisense LuxI
 
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| Part
 
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| Memory
 
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| In use
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145014'''
 
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| /
 
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| T7 RNA Polymerase with N-terminal UmuD degradation tag
 
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| Part
 
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| Filter
 
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| In use
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145015'''
 
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| /
 
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| GFP with LVA tag
 
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| Part
 
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| Ouput
 
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| In use
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145016'''
 
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| /
 
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| Intermediate TetR-generator
 
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| Composite part
 
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| Input
 
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| In use
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145100'''
 
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| '''K145205'''
 
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| OmpF PoPS -> GFP
 
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| Composite part
 
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| Output
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145101'''
 
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| '''K145110'''
 
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| OmpF PoPS -> deactivate constitutive antisense LuxI
 
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| Composite part
 
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| Memory
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145102'''
 
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| '''K145215'''
 
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| OmpF PoPS -> RiboKey3d
 
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| Composite part
 
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| Filter
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145103'''
 
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| '''K145216'''
 
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| T7 with UmuD derived tag under constitutive promotor + RiboLock3d
 
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| Composite Part
 
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| Filter
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145104'''
 
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| '''K145220'''
 
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| T7 PoPS + RiboLock -> LacI -> inhibition of LuxI
 
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| Composite part
 
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| Inverter
 
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| Discarded
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145105'''
 
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| '''K145225'''
 
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| cI under T7 and P<sub>R</sub> dual promotor
 
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| Composite part
 
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| Pulse Generator
 
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| Discarded
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145106'''
 
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| /
 
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| cI P<sub>RM</sub> PoPS -> Lactonase
 
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| Composite part
 
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| Pulse Generator
 
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| Unknown
 
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|- style="background:#e0ffff; color:grey"
 
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| '''K145107'''
 
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| '''K145117'''
 
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| Lactonase pulse generator
 
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| Composite part
 
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| Pulse Generator
 
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| Discarded
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145108'''
 
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| /
 
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| Constitutive PoPS -> LuxR
 
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| Composite part
 
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| Cell death
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145109'''
 
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| /
 
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| Hybrid P22 c2 - luxP<sub>R</sub> PoPS -> ccdB
 
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| Composite part
 
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| Cell death
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145110'''
 
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| /
 
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| Cell death mechanism
 
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| Composite part
 
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| Cell death
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145111'''
 
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| /
 
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| OmpF PoPS -> deactivate constitutive antisense LuxI
 
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| Composite part
 
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| Memory
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145112'''
 
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| /
 
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| cI under T7 and PR<sub>R</sub> dual promotor
 
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| Composite part
 
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| Pulse generator
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145113'''
 
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| /
 
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| Constitutive PoPS -> TetR
 
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| ?
 
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| ?
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145115'''
 
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| /
 
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| cI under T7 and PRR dual promotor
 
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| Composite part
 
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| Pulse generator
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145117'''
 
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| /
 
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| Lactonase pulse generator
 
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| Composite part
 
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| Pulse generator
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145150'''
 
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| /
 
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| Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
 
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| Part
 
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| CellDeath
 
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| In use
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145151'''
 
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| /
 
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| ccdB coding region
 
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| Part
 
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| CellDeath
 
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| In use
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145152'''
 
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| /
 
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| Hybrid promoter: P22 c2 , LacI NOR gate
 
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| Part
 
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| ?
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145200'''
 
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| /
 
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| INPUT lightsensor (DISCARDED)
 
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| Composite Part
 
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| Input
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145201'''
 
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| /
 
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| INPUT TetR generator
 
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| Composite Part
 
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| INPUT
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145205'''
 
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| /
 
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| OUTPUT GFP under TetR pomoter
 
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| Composite Part
 
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| OUTPUT
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145210'''
 
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| /
 
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| MEMORY
 
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| Composite Part
 
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| MEMORY
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145211'''
 
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| /
 
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| MEMORY OUTPUT asLuxI
 
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| Composite Part
 
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| MEMORY
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145215'''
 
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| /
 
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| FILTER Key
 
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| Composite Part
 
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| FILTER
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145216'''
 
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| /
 
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| FILTER T7 RNA pol
 
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| Composite Part
 
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| FILTER
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145217'''
 
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| /
 
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| FILTER Complete
 
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| Composite Part
 
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| FILTER
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145220'''
 
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| /
 
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| INVERTED TIMER
 
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| Composite Part
 
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| INVERTIMER
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145225'''
 
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| /
 
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| RESET lactonase
 
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| Composite Part
 
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| RESET
 
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| Unknown
 
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|- style="background:#e0ffff; color:black"
 
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| '''K145230'''
 
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| /
 
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| CELL DEATH
 
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| Composite Part
 
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| CELLDEATH
 
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| Unknown
 
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|}
 
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==The Big Scheme of Used Parts==
 
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[https://static.igem.org/mediawiki/2008/6/6d/THE_BIG_SCHEME.xls The Big Scheme]
 

Latest revision as of 00:10, 27 October 2008

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