Team:NYMU-Taipei/Project/Time Regulation/Results of Cyanoxilator
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Blackrabbit (Talk | contribs) (New page: {{:Team:NYMU-Taipei/Header}} === Extraction of the genomic DNA of S. elongatus PCC 7942=== {| | Image:NYMU 20080826 PCC7942 genomic DNA with 1kb marker.JPG | * tube 1: 101.3 ng/uL (26...) |
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* comment: | * comment: | ||
- | successfully pcr back-RpaA and front-SasA; however they are too weak and we | + | successfully pcr back-RpaA and front-SasA; however they are too weak and we encountered a little bit of trouble doing overlap extension PCR |
* action: | * action: | ||
**gel extraction | **gel extraction | ||
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|[[Image:NYMU 0929GFPm.jpg]] | |[[Image:NYMU 0929GFPm.jpg]] | ||
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- | * The M9 | + | * The M9 does not seems to have enough nutrition for ''E. coli'' to grow. |
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|10/4 | |10/4 | ||
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|[[Image:NYMU 20080930 od lb.jpg|500px]] | |[[Image:NYMU 20080930 od lb.jpg|500px]] | ||
|[[Image:NYMU 0930GFPm.jpg]] | |[[Image:NYMU 0930GFPm.jpg]] | ||
- | |* For the 3 hr measure, the plate was not | + | |* For the 3 hr measure, the plate was not washed carefully, so there is a large background. |
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|10/4 | |10/4 | ||
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==Discussion of the reporting== | ==Discussion of the reporting== | ||
- | * | + | * Is the freshness of the bacteria important to GFP expression? If so, we should use freshly streaked plates. |
- | * The white and black plates must | + | * The white and black plates must be cleaned carefully, otherwise there would be too much background. |
- | *pBF | + | *pBF seemes to grow much faster than the others. |
{{:Team:NYMU-Taipei/Footer}} | {{:Team:NYMU-Taipei/Footer}} |
Revision as of 00:31, 30 October 2008
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Contents |
Extraction of the genomic DNA of S. elongatus PCC 7942
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PCR of RpaA and SasA
Reporting assay
Discussion of the reporting
- Is the freshness of the bacteria important to GFP expression? If so, we should use freshly streaked plates.
- The white and black plates must be cleaned carefully, otherwise there would be too much background.
- pBF seemes to grow much faster than the others.