Team:Paris/August 11

From 2008.igem.org

(Difference between revisions)
(→Culture of ligation transformants)
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{|| Border="1"
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|align="center"|'''Ligation'''
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| colspan="8" align="center"|L128
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| colspan="8" align="center"|L129
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| colspan="8" align="center"|L130
 +
|-
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|align="center"|'''Name'''
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| colspan="8" align="center"|pFlgA
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| colspan="8" align="center"|pFlgB
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| colspan="8" align="center"|pFlhB
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|-
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|align="center"|''Clone N°'''
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|align="center"|1
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|align="center"|2
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|align="center"|3
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|align="center"|4
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|align="center"|1
 +
|align="center"|2
 +
|align="center"|6
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|align="center"|7
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|align="center"|1
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|align="center"|2
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|align="center"|7
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|align="center"|8
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|-
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|align="center"|'''Red fluorescence'''
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|align="center"|yes
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|align="center"|yes
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|align="center"|no
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|align="center"|no
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|align="center"|yes
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|align="center"|yes
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|align="center"|no
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|align="center"|no
 +
|align="center"|yes
 +
|align="center"|no
 +
|align="center"|no
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|align="center"|no
|}
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Revision as of 16:12, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
  • 37°C overnight
Ligation Name Clone N° Red Fluorescence
L128 pFlgA
L129 pFlgB
L130 pFlhB
L131 pFlhDC
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N°' 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no
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