Team:Paris/August 11
From 2008.igem.org
(→PCR verification/Analysis) |
(→Culture of ligation transformants) |
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==> '''Conclusion :''' We need to repeat the experiments. | ==> '''Conclusion :''' We need to repeat the experiments. | ||
- | ==Culture of ligation transformants== | + | ==Culture of ligation transformants (pFlgA, pFlgB and pFlhB)== |
*4 clones of each transformation were cultured in '''7,5 mL LB + ampicilline'''. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8). | *4 clones of each transformation were cultured in '''7,5 mL LB + ampicilline'''. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8). |
Latest revision as of 16:11, 18 August 2008
TransformationAll the ligations were transformed according transformation for Top10 protocol
PCRWe performed PCR on to amplify the sequence in order to have enough amount of DNA to carry out the following of our experiments.
PCR amplificationProtocol
For each sample, 1 µl dNTP
PCR verification/AnalysisAfter the PCR :
ladder : 10µl ladder 1 kb
ladder : 10µl ladder 100 bp
Culture of ligation transformants (pFlgA, pFlgB and pFlhB)
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