Team:Paris/August 13
From 2008.igem.org
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+ | ==Assay to purify a PCR product using the Qiaquick Gel Extraction kit== | ||
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+ | *sample used: yesterday PCR product of L130.8 (~40 µL) | ||
+ | *protocol used: Qiagen PCR purification kit | ||
+ | *use of buffer QG (Gel Extraction kit) instead of buffer PBI (PCR purification kit) | ||
+ | *elution in 30 µL of buffer EB | ||
+ | *30 µL of purified product + 12 µL of loading blue | ||
+ | *electrophoresis, 10 µL loaded, --> see Gel 1, well n°8 | ||
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+ | ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! | ||
==PCR screening== | ==PCR screening== |
Revision as of 12:29, 14 August 2008
Sequencing Minipreps we did yesterday
Minipreps: Plasmid extraction
Glycerol Stocks
Assay to purify a PCR product using the Qiaquick Gel Extraction kit
==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! PCR screening4 more transformants of L130 (pFlhB into J61002) are screened by PCR.
Electrophoresis
Promoter Characterization: work plan |