Team:Paris/August 13
From 2008.igem.org
(Difference between revisions)
(→PCR screening) |
(→Assay to purify a PCR product using the Qiaquick Gel Extraction kit) |
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*elution in 30 µL of buffer EB | *elution in 30 µL of buffer EB | ||
*30 µL of purified product + 12 µL of loading blue | *30 µL of purified product + 12 µL of loading blue | ||
- | *electrophoresis, 10 µL loaded | + | *electrophoresis, 10 µL loaded |
+ | --> see Gel 1, well n°8 | ||
==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! | ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! |
Revision as of 12:30, 14 August 2008
Sequencing Minipreps we did yesterday
Minipreps: Plasmid extraction
Glycerol Stocks
Assay to purify a PCR product using the Qiaquick Gel Extraction kit
--> see Gel 1, well n°8 ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! PCR screening4 more transformants of L130 (pFlhB into J61002) are screened by PCR.
Electrophoresis
Promoter Characterization: work plan |