Team:Paris/August 13
From 2008.igem.org
(Difference between revisions)
(→PCR screening) |
(→Electrophoresis) |
||
Line 298: | Line 298: | ||
|align=center|positive control | |align=center|positive control | ||
|align=center|negative control | |align=center|negative control | ||
- | |align=center|L130.3 | + | |align=center|'''L130.3''' |
- | |align=center|L130.4 | + | |align=center|'''L130.4''' |
- | |align=center|L130.5 | + | |align=center|'''L130.5''' |
- | |align=center|L130.6 | + | |align=center|'''L130.6''' |
|align=center|PCR product (L130.8) purified by the Qiaquick Gel Extraction kit | |align=center|PCR product (L130.8) purified by the Qiaquick Gel Extraction kit | ||
|- | |- | ||
Line 318: | Line 318: | ||
|align=center|1,161 kb | |align=center|1,161 kb | ||
|align=center|0 kb | |align=center|0 kb | ||
- | | | + | |colspan="4"|<center>'''1,338 kb'''</center> |
- | + | ||
- | + | ||
- | + | ||
|align=center|doesn't matter | |align=center|doesn't matter | ||
|- | |- |
Revision as of 12:32, 14 August 2008
Sequencing Minipreps we did yesterday
Minipreps: Plasmid extraction
Glycerol Stocks
Assay to purify a PCR product using the Qiaquick Gel Extraction kit
--> see Gel 1, well n°8 ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! PCR screening4 more transformants of L130 (pFlhB into J61002) are screened by PCR.
Electrophoresis
Promoter Characterization: work plan |