Team:Paris/August 14
From 2008.igem.org
(Difference between revisions)
(→Results of the PCR of the wonderful promoter of fliL) |
(→PCR screening of cloning of E0240, flhD, flhC and pflhDC+flhDC) |
||
Line 132: | Line 132: | ||
*positive control 2: pSB3K3 | *positive control 2: pSB3K3 | ||
*negative control: no template | *negative control: no template | ||
+ | |||
+ | |||
+ | Is it possible to have a picture please !! |
Revision as of 16:34, 15 August 2008
Results of the PCR of the wonderful promoter of fliL
Remarks: We observe that the bands are curved, we suppose that the wells were not very clean. The size of fliL is good, we will digest it and ligate it today. Digestion and Ligation of the wonderful promoter of fliLAs the primer used to amplify the promoter of fliL had only two nucleotides after the restriction sites, we tried the two digestions possible : EcoRI + SpeI and XbaI + PstI. DigestionProtocol :
Washing of the digestionsWe washed the DNA following the standard protocol. Ligation
PCR screening of cloning of E0240, flhD, flhC and pflhDC+flhDC
Is it possible to have a picture please !! |