Team:Paris/August 15
From 2008.igem.org
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=='''PCR amplification of flhDC and its promoter'''== | =='''PCR amplification of flhDC and its promoter'''== | ||
+ | As we did not manage to amplofy flhDC or its promoter alone, we decided to amplify both flhDC and its promoter. From the big amplicon, we will try to get the promoter alone. | ||
==='''List of PCRs'''=== | ==='''List of PCRs'''=== |
Revision as of 17:59, 16 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells standard protocol. PCR amplification of flhDC and its promoterAs we did not manage to amplofy flhDC or its promoter alone, we decided to amplify both flhDC and its promoter. From the big amplicon, we will try to get the promoter alone. List of PCRs
ProtocolWe followed the standard protocol of amplification in Two steps. PCR program used : PHUSION2 ResultsSettings Gel 1%
DigestionsDigestionMeasurement of concentration of minipreps
DigestionLigation
Analysis of yesterday PCR screening
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
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