Team:Paris/August 18
From 2008.igem.org
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(→Analysis of the cloning of OmpR and EnvZ) |
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<br>3 clones of L133 and 3 clones of L134 were analysed. | <br>3 clones of L133 and 3 clones of L134 were analysed. | ||
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | <br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | ||
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+ | =='''Miniprep and stock glycerol of L151'''== |
Revision as of 14:51, 19 August 2008
Analysis of the cloning of OmpR and EnvZPCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th.
Miniprep and stock glycerol of L151 |