Team:Paris/August 18
From 2008.igem.org
(Difference between revisions)
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=='''Miniprep and stock glycerol of L151'''== | =='''Miniprep and stock glycerol of L151'''== | ||
*[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Stock number''' | ||
+ | |'''Ligation name''' | ||
+ | |'''Biobricks''' | ||
+ | |'''Description''' | ||
+ | |- | ||
+ | |S162.1 | ||
+ | |L151.1 | ||
+ | |rowspan="3" |[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
+ | |rowspan="3" |rbs-LasR - Double terminator | ||
+ | |- | ||
+ | |S162.2 | ||
+ | |L151.2 | ||
+ | |- | ||
+ | |S162.3 | ||
+ | |L151.3 | ||
+ | |} | ||
+ | |||
+ | |||
*[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]] | *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]] | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Miniprep Name''' | ||
+ | |'''Ligation name''' | ||
+ | |'''Antibio''' | ||
+ | |'''Biobricks''' | ||
+ | |'''Description''' | ||
+ | |- | ||
+ | |MP162.1 | ||
+ | |L151.1 | ||
+ | |rowspan="3" |Kan | ||
+ | |rowspan="3" |[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
+ | |rowspan="3" |rbs-LasR - Double terminator | ||
+ | |- | ||
+ | |MP162.2 | ||
+ | |L151.2 | ||
+ | |- | ||
+ | |MP162.3 | ||
+ | |L152.3 | ||
+ | |} |
Revision as of 15:01, 19 August 2008
Analysis of the cloning of OmpR and EnvZPCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th.
Miniprep and stock glycerol of L151
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