Team:Paris/August 18
From 2008.igem.org
(Difference between revisions)
(→Analysis of the cloning of OmpR* and EnvZ*) |
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{{Paris/Calendar_Links|August 17|August 19}} | {{Paris/Calendar_Links|August 17|August 19}} | ||
- | =Analysis of the cloning of OmpR | + | =Analysis of the cloning of OmpR and EnvZ= |
==PCR screening== | ==PCR screening== | ||
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*0,5 µL O19 | *0,5 µL O19 | ||
*12 µL water | *12 µL water | ||
- | We wanted to analyse the transformants of the ligations L133 (OmpR | + | We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th. |
<br>3 clones of L133 and 3 clones of L134 were analysed. | <br>3 clones of L133 and 3 clones of L134 were analysed. | ||
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | <br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | ||
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|MP162.3 | |MP162.3 | ||
|L151.3 | |L151.3 | ||
+ | |} | ||
+ | |||
+ | =='''Digestion of MP162'''== | ||
+ | ==='''Measurement concentration MPs 162'''=== | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Miniprep Name''' | ||
+ | |'''Concentration (µg/mL)''' | ||
+ | |'''Ratio 260/280''' | ||
+ | |- | ||
+ | |MP162.1 | ||
+ | |151 | ||
+ | |1.71 | ||
+ | |- | ||
+ | |MP162.2 | ||
+ | |172 | ||
+ | |1.73 | ||
+ | |- | ||
+ | |MP162.3 | ||
+ | |149 | ||
+ | |1.73 | ||
|} | |} |
Revision as of 15:11, 19 August 2008
Analysis of the cloning of OmpR and EnvZPCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th.
Miniprep and stock glycerol of L151
Digestion of MP162Measurement concentration MPs 162
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