Team:Paris/August 18
From 2008.igem.org
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=='''Miniprep and stock glycerol of L151 (rbs-LasR - Double terminator)'''== | =='''Miniprep and stock glycerol of L151 (rbs-LasR - Double terminator)'''== | ||
*[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | ||
+ | *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]] | ||
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{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
+ | |'''Miniprep number''' | ||
|'''Stock number''' | |'''Stock number''' | ||
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|'''Ligation name''' | |'''Ligation name''' | ||
|'''Antibio''' | |'''Antibio''' | ||
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|MP162.1 | |MP162.1 | ||
+ | |S162.1 | ||
|L151.1 | |L151.1 | ||
|rowspan="3" |Kan | |rowspan="3" |Kan | ||
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|MP162.2 | |MP162.2 | ||
+ | |S162.2 | ||
|L151.2 | |L151.2 | ||
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|MP162.3 | |MP162.3 | ||
+ | |S162.3 | ||
|L151.3 | |L151.3 | ||
|} | |} | ||
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=Construction of : promotor-rbs-LasR - Dble ter= | =Construction of : promotor-rbs-LasR - Dble ter= |
Revision as of 13:36, 30 August 2008
Analysis of the cloning of OmpR* and EnvZ*PCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th.
Construction of rbs-LasR-dble terMiniprep and stock glycerol of L151 (rbs-LasR - Double terminator)
Construction of : promotor-rbs-LasR - Dble terDigestionMeasurement of the concentration of MPs 162
Digestion
Gel Extraction
Ligation
Culture of stable strains with biobricks 2008
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