Team:Paris/August 18
From 2008.igem.org
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*[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]] | *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]] | ||
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Revision as of 13:37, 30 August 2008
Analysis of the cloning of OmpR* and EnvZ*PCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th.
Construction of rbs-LasR-dble terMiniprep and stock glycerol of L151 (rbs-LasR - Double terminator)
Construction of : promotor-rbs-LasR - Dble terDigestionMeasurement of the concentration of MPs 162
Digestion
Gel Extraction
Ligation
Culture of stable strains with biobricks 2008
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