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Team:Paris/August 18
From 2008.igem.org
(Difference between revisions)
(New page: {{Paris/Calendar_Links|August 17|August 19}} =Analysis of the cloning of OmpR and EnvZ= ==PCR screening== *12,5 microL Quick load PCR Mix *0,5 microL O18 *0,5 microL O19 *12 microL wate...) |
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==PCR screening== | ==PCR screening== | ||
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*positive control: S158 (pSB3K3) | *positive control: S158 (pSB3K3) | ||
*negative control: no template | *negative control: no template | ||
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*elongation time: 1 min 30 | *elongation time: 1 min 30 | ||
*primers: O18 & O19 | *primers: O18 & O19 | ||
| + | '''Reaction mixture''' (25 microL total volume) | ||
| + | *12,5 microL Quick load PCR Mix | ||
| + | *0,5 microL O18 | ||
| + | *0,5 microL O19 | ||
| + | *12 microL water | ||
<br>We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august | <br>We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august | ||
3 clones of L133 and 3 clones of L134 were analysed. | 3 clones of L133 and 3 clones of L134 were analysed. | ||
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | <br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | ||
Revision as of 18:18, 18 August 2008
Analysis of the cloning of OmpR and EnvZPCR screening
Reaction mixture (25 microL total volume)
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