Team:Paris/August 18

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(Difference between revisions)
(→PCR screening)
(→PCR screening)
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*elongation time: 1 min 30
*elongation time: 1 min 30
*primers: O18 & O19
*primers: O18 & O19
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'''Reaction mixture''' (25 microL total volume)
+
'''Reaction mixture''' (25 µL total volume)
-
*12,5 microL Quick load PCR Mix
+
*12,5 µL Quick load PCR Mix
-
*0,5 microL O18
+
*0,5 µL O18
-
*0,5 microL O19
+
*0,5 µL O19
-
*12 microL water
+
*12 µL water
-
<br>We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august
+
<br>We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th and 10th.
3 clones of L133 and 3 clones of L134 were analysed.
3 clones of L133 and 3 clones of L134 were analysed.
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline.
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline.

Revision as of 18:21, 18 August 2008

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Analysis of the cloning of OmpR and EnvZ

PCR screening

  • positive control: S158 (pSB3K3)
  • negative control: no template
  • screening PCR programm
  • elongation time: 1 min 30
  • primers: O18 & O19

Reaction mixture (25 µL total volume)

  • 12,5 µL Quick load PCR Mix
  • 0,5 µL O18
  • 0,5 µL O19
  • 12 µL water


We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th and 10th. 3 clones of L133 and 3 clones of L134 were analysed.
Each clone tested was then cultured in 7,5 mL LB + ampicilline.

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