Team:Paris/August 18
From 2008.igem.org
(Difference between revisions)
(→Analysis of the cloning of OmpR and EnvZ) |
(→Analysis of the cloning of OmpR and EnvZ) |
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*0,5 µL O19 | *0,5 µL O19 | ||
*12 µL water | *12 µL water | ||
- | <br>We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/ | + | <br>We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th. |
<br>3 clones of L133 and 3 clones of L134 were analysed. | <br>3 clones of L133 and 3 clones of L134 were analysed. | ||
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | <br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. |
Revision as of 18:23, 18 August 2008
Analysis of the cloning of OmpR and EnvZPCR screening
Reaction mixture (25 µL total volume)
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