Team:Paris/August 18

From 2008.igem.org

(Difference between revisions)
(→Miniprep and stock glycerol of L151)
(→Miniprep and stock glycerol of L151)
Line 22: Line 22:
=='''Miniprep and stock glycerol of L151'''==
=='''Miniprep and stock glycerol of L151'''==
*[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]]
*[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Stock number'''
 +
|'''Ligation name'''
 +
|'''Biobricks'''
 +
|'''Description'''
 +
|-
 +
|S162.1
 +
|L151.1
 +
|rowspan="3" |[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 +
|rowspan="3" |rbs-LasR - Double terminator
 +
|-
 +
|S162.2
 +
|L151.2
 +
|-
 +
|S162.3
 +
|L151.3
 +
|}
 +
 +
*[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]]
*[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Miniprep Name'''
 +
|'''Ligation name'''
 +
|'''Antibio'''
 +
|'''Biobricks'''
 +
|'''Description'''
 +
|-
 +
|MP162.1
 +
|L151.1
 +
|rowspan="3" |Kan
 +
|rowspan="3" |[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
 +
|rowspan="3" |rbs-LasR - Double terminator
 +
|-
 +
|MP162.2
 +
|L151.2
 +
|-
 +
|MP162.3
 +
|L152.3
 +
|}

Revision as of 15:01, 19 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Analysis of the cloning of OmpR and EnvZ

PCR screening

  • positive control: S158 (pSB3K3)
  • negative control: no template
  • screening PCR programm
  • elongation time: 1 min 30
  • primers: O18 & O19

Reaction mixture (25 µL total volume)

  • 12,5 µL Quick load PCR Mix
  • 0,5 µL O18
  • 0,5 µL O19
  • 12 µL water

We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th.
3 clones of L133 and 3 clones of L134 were analysed.
Each clone tested was then cultured in 7,5 mL LB + ampicilline.


Miniprep and stock glycerol of L151

Stock number Ligation name Biobricks Description
S162.1 L151.1 Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-LasR - Double terminator
S162.2 L151.2
S162.3 L151.3


Miniprep Name Ligation name Antibio Biobricks Description
MP162.1 L151.1 Kan Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-LasR - Double terminator
MP162.2 L151.2
MP162.3 L152.3
Retrieved from "http://2008.igem.org/Team:Paris/August_18"