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Team:Paris/August 18
From 2008.igem.org
(Difference between revisions)
(→Digestion) |
(→Analysis of the cloning of OmpR and EnvZ) |
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{{Paris/Calendar_Links|August 17|August 19}} | {{Paris/Calendar_Links|August 17|August 19}} | ||
| - | =Analysis of the cloning of OmpR and EnvZ= | + | =Analysis of the cloning of OmpR* and EnvZ*= |
==PCR screening== | ==PCR screening== | ||
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*0,5 µL O19 | *0,5 µL O19 | ||
*12 µL water | *12 µL water | ||
| - | We wanted to analyse the transformants of the ligations L133 (OmpR in pSB1A2) and L134 (EnvZ in pSB1A2) from the experience of august 9th/11th. | + | We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th. |
<br>3 clones of L133 and 3 clones of L134 were analysed. | <br>3 clones of L133 and 3 clones of L134 were analysed. | ||
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | <br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | ||
Revision as of 15:36, 19 August 2008
Analysis of the cloning of OmpR* and EnvZ*PCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th.
Miniprep and stock glycerol of L151
Digestion of MP162Measurement of the concentration of MPs 162
Digestion
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