Team:Paris/August 18
From 2008.igem.org
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=='''Miniprep and stock glycerol of L151 (rbs-LasR - Double terminator)'''== | =='''Miniprep and stock glycerol of L151 (rbs-LasR - Double terminator)'''== | ||
*[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] |
Revision as of 13:33, 30 August 2008
Analysis of the cloning of OmpR* and EnvZ*PCR screening
Reaction mixture (25 µL total volume)
We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th.
Construction of rbs-LasR-dble terMiniprep and stock glycerol of L151 (rbs-LasR - Double terminator)
Construction of : promotor-rbs-LasR - Dble terDigestion of MP162 (rbs-LasR - Dble ter)Measurement of the concentration of MPs 162
Digestion
Gel Extraction
Ligation
Culture of stable strains with biobricks 2008
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