Team:Paris/August 19
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==Ligation== | ==Ligation== | ||
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==Transformation== | ==Transformation== |
Revision as of 14:42, 21 August 2008
Screening of the cloning of OmpR*, EnvZ* and FlhDC+promotorElectrophoresis
Minipreps and glycerol stock
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The PCR screening of the transformants L139 and L142 of august 15th revealed several bands for a given clone including one band appearing at the right size.
In order to check these 2 hypothesis and to isolate (if it is possible) the right clone (containing the plasmid with the insert). We decided to spread the "clone" in question in a LB plate in order to carry out a PCR screening on single colonies.
Promoter characterization plasmidsLigation
TransformationDigestionMeasurement of concentration of miniprepsto be modified standard protocol
Digestionto be modified
We had a problem with a gel and we lost these digestions. |